| 侵染观赏百合的黄瓜花叶病毒提纯和抗血清制备 |
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| 引用本文： | 梁巧兰,魏列新,徐秉良.侵染观赏百合的黄瓜花叶病毒提纯和抗血清制备[J].甘肃农业大学学报,2007,42(1):59-62. |
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| 作者姓名： | 梁巧兰 魏列新 徐秉良 |
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| 作者单位： | 甘肃农业大学草业学院,甘肃,兰州,730070 |
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| 基金项目： | 国家科技部项目(2001EA86005)，兰州市科委项目(03-1-39)共同资助 |
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| 摘 要： | 采用PEG沉淀并结合差速离心技术,获得了精提纯的侵染观赏百合的黄瓜花叶病毒(CMV-Li).提纯病毒具有典型的核蛋白吸收峰曲线.OD260 nm/OD280 nm=1.236.将提纯病毒免疫兔子制备得CMV-Li抗血清.经微量沉淀法和间接ELISA法测定,其效价分别为1∶1024、1∶4096.两种方法相比,间接ELISA具有灵敏度高、特异反应强等优点.
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| 关 键 词： | 观赏百合 黄瓜花叶病毒 提纯 抗血清制备 |
| 文章编号： | 1003-4315（2007）01-0059-04 |
| 修稿时间： | 2006-04-21 |
Preparation of antiserum to cucumbor mosaic virus infected ornamental lily |
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| LIANG Qiao-lan,WEI Lie-xin,XU Bing-liang.Preparation of antiserum to cucumbor mosaic virus infected ornamental lily[J].Journal of Gansu Agricultural University,2007,42(1):59-62. |
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| Authors: | LIANG Qiao-lan WEI Lie-xin XU Bing-liang |
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| Institution: | College of Prataculture, Gansu Agricultural University, Lanzhou 730070, China |
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| Abstract: | The cucumbor mosaic virus infected lily was purified from leaves of the diseased Phaseolus vulgaris plant inocultated artificially with CMV-Li by the method of differential centrifugation in combination with PEG6000.The purified CMV-Li particles were identified by electron microscope observation and ultra-violet scanning analysis and had typical nucler protein absorb curve,the ratio of OD260 nm to OD280 nm was 1.236.Then purified CMV-Li preparation was injected into a rabbit to produce an antiserum to CMV-Li.The titer of antiserum was different by Miroprecipitation tested and indirected-ELISA,its 1∶1024 and 1∶4096.By compared,the sensibility and the distinctive reaction were the highest of the indirected-ELISA. |
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| Keywords: | ornamental lily cucumber mosaic virus infected lily(CMV-Li) purification antiserum prepare |
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