| 不同培养液及血清浓度对绵羊孤雌生殖胚胎发育的影响 |
| |
| 引用本文： | 张宏,刘云海,倪和民,杨飞鸽,刘烈琴,薛君忠,郭勇.不同培养液及血清浓度对绵羊孤雌生殖胚胎发育的影响[J].中国畜牧兽医,2007,34(7):32-34. |
| |
| 作者姓名： | 张宏 刘云海 倪和民 杨飞鸽 刘烈琴 薛君忠 郭勇 |
| |
| 作者单位： | 北京农学院动物科学技术系，北京 102206 |
| |
| 基金项目： | 北京市教委科技发展计划项目;北京市市属市管高等院校中青年骨干教师培养计划;北京市农业新技术重点实验室部分资助项目 |
| |
| 摘 要： | 体外成熟的绵羊卵母细胞，经5 μmol/L A23187 5 min和2 mmol/L 6-DMAP 4 h激活后，分别在SOFaa、M-199两种培养液中进行培养，试验比较了SOFaa、M-199液分别添加BSA和不同浓度（10%、20%）胎牛血清对绵羊孤雌生殖胚体外发育的影响。M-199+FCS组的卵裂率显著低于SOFaa+BSA和SOFaa+FCS组（P<0.05），SOFaa+BSA组的囊胚率显著低于M-199+BSA、SOFaa+FCS、M-199+FCS三组（P<0.05）；SOFaa+10% FCS与SOFaa+20% FCS组的卵裂率及囊胚率均无显著差异，M-199+10% FCS和M-199+20% FCS组的卵裂率及囊胚率间均无显著差异。结果表明：SOFaa比M-199更适合绵羊孤雌生殖胚体外发育，此外添加10%的FCS即可达到较好的培养效果。
|
| 关 键 词： | 绵羊 孤雌胚 体外培养 |
| 文章编号： | 1671-7236（2007）07-0032-03 |
| 修稿时间： | 2006-12-11 |
Effect of Media and Serum Concentrations on Development of Ovine Parthenogenetic Embryos |
| |
| ZHANG Hong,LIU Yun-hai,NI He-min,YANG Fei-ge,LIU Lie-qin,XUE Jun-zhong,GUO Yong.Effect of Media and Serum Concentrations on Development of Ovine Parthenogenetic Embryos[J].China Animal Husbandry & Veterinary Medicine,2007,34(7):32-34. |
| |
| Authors: | ZHANG Hong LIU Yun-hai NI He-min YANG Fei-ge LIU Lie-qin XUE Jun-zhong GUO Yong |
| |
| Institution: | Department of Animal Science and Technology in Beijing University of Agriculture, Beijing 102206, China |
| |
| Abstract: | Matured ovine oocytes were activated by 5 μmol/L A23187 for 5 min and then 2 mmol/L 6-DMAP for 4 h,afterwards cultured in SOFaa and M-199 medium supplemented with BSA and serum of different concentrations, respectively. The cleavage rate of group M-199+FCS(29.9%) was significantly lower than SOFaa+BSA(52.7%) and SOFaa+FCS (52.8%) groups (P<0.05). And the blastocystrate of SOFaa+BSA(0.8%) group was significantily lower than M-199+BSA(7.6%),SOFaa+FCS(11.8%),M-199+FCS(10.2%) groups (P<0.05). The cleavage rate and blastocyst rate of oocyte treated with SOFaa+10% FCS（50.7%,11.3%）and SOFaa+20% FCS（59.2%,9.9%）were not significantly different, and those data of M-199+10% FCS（29.6%,5.6%） and M-199+20% FCS（32.4%,9.9%）were also same. These results showed that SOFaa medium is suitable for the development of ovine parthenogenetic embryos cultured in vitro , and SOFaa added with10% FCS can improve its function on those IVC embryos. |
| |
| Keywords: | ovine parthenogenesis in vitro culture |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
