| 致病性嗜水气单胞菌耐喹诺酮类药物菌株的分离鉴定 |
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| 引用本文： | 卢强,郑伟,刘明远,张雅斌,韩文瑜.致病性嗜水气单胞菌耐喹诺酮类药物菌株的分离鉴定[J].中国兽医学报,2003,23(4):331-333. |
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| 作者姓名： | 卢强 郑伟 刘明远 张雅斌 韩文瑜 |
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| 作者单位： | 1. 解放军军需大学,军事兽医系,吉林,长春,130062
2. 吉林省水产科学研究所,吉林,长春,130051 |
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| 基金项目： | 国家自然科学基金资助项目(30070587);吉林省自然科学基金资助项目(20020648) |
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| 摘 要： | 对吉林省内12个水域采集和送检的66尾病鱼进行了细菌分离鉴定，共检出46株嗜水气单胞菌疑似菌，通过生化试验结合PCR扩增气溶素基因Aer，确定其中22株为致病性嗜水气单胞菌。进一步采用纸片扩散法和双倍稀释法测定嗜水气单胞菌分离株对多种抗生素的耐药性，其中有4株对喹诺酮类药物耐药，并且为交叉耐药，耐药菌检出率为18．2％，纸片扩散法检测其对喹诺酮类药物的抑菌圈均小于19mm。将病料中分离的8株嗜水气单胞菌负染后电镜观察，耐药菌表面有许多大小不同的球形结构，而敏感菌表面则为细丝状菌毛结构。
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| 关 键 词： | 致病性嗜水气单胞菌 喹诺酮类药物 耐药菌株 分离 鉴定 表面结构 球形结构 细丝状菌毛结构 水产细菌病 |
| 文章编号： | 1005-4545(2003)04-0331-02 |
| 修稿时间： | 2002年6月23日 |
Isolation and Identification of Quinolone Resistant Strains of Pathogenic Aeromonas hydrophil |
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| LU Qiang ,ZHENG Wei ,LIU Ming yuan ,ZHANG Ya bing ,HAN Wen yu.Isolation and Identification of Quinolone Resistant Strains of Pathogenic Aeromonas hydrophil[J].Chinese Journal of Veterinary Science,2003,23(4):331-333. |
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| Authors: | LU Qiang ZHENG Wei LIU Ming yuan ZHANG Ya bing HAN Wen yu |
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| Institution: | LU Qiang 1,ZHENG Wei 2,LIU Ming yuan 1,ZHANG Ya bing 2,HAN Wen yu 1 |
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| Abstract: | Bacterial pathogens were isolated from fishes collected from 12 water regions in Jilin province.Biochemical tests and amplification of aer gene with PCR were utilized to identify the Aeromonas hydrophil(Ah.) isolates.Among forty six isolates tested,22 strains were identified as A.h and 4 drug resistant strains were identified by routine agar diffusion and disk diffusion test assays.Norfloxacin,ciprofloxacin and ofloxacin resistance were verified according to National Committee for Clinical Laboratory Standards.The identified strains,including 6 susceptible and 2 resistant strains were cultured in LB,washed with physiological saline,centrifugated at room temperature,dyed with phosphorus wolfram acid,observed on electron microscope and compared their surface structure.Many small globule,which were different in size,were observed on the surface of the 2 drug resistant strains,but not on the surface of the 6 susceptible strains.The isolation of quinolones resistant Aeromonas hydrophil strains in Northern China indicates that it is important to establish a system of identification of drug resistant strains in environment. |
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| Keywords: | Aeromonas hydrophil quinolones drug resistant bacteria surface structure |
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