| 中国湛江市和哈尔滨市褐家鼠种群遗传结构及其年度变化特征 |
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| 引用本文： | 陈燕,马晓慧,王大伟,李宁,姚丹丹,冯志勇,刘晓辉,宋英.中国湛江市和哈尔滨市褐家鼠种群遗传结构及其年度变化特征[J].植物保护学报,2021,48(2):449-457. |
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| 作者姓名： | 陈燕 马晓慧 王大伟 李宁 姚丹丹 冯志勇 刘晓辉 宋英 |
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| 作者单位： | 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193;广东省农业科学院植物保护研究所, 植物保护新技术重点实验室, 广州 510640 |
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| 基金项目： | 国家自然科学基金（31871986，31401761），北京市自然科学基金（5154033） |
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| 摘 要： | 为探索褐家鼠Rattus norvegicus地理种群的遗传结构及其年度变化特点，本研究以广东省湛江市的褐家鼠指名亚种和黑龙江省哈尔滨市的褐家鼠东北亚种为主要研究对象，结合我国及世界其他褐家鼠种群的D-loop序列分析这2个褐家鼠地理种群间D-loop序列的遗传分化情况及系统进化关系，重点分析2008—2015年褐家鼠湛江种群和哈尔滨种群D-loop单倍型的年度频率变化特点。结果表明，褐家鼠湛江种群和哈尔滨种群共有32种不同的单倍型，其中有11种单倍型是2个种群共有的，有4种单倍型仅在湛江种群中出现，有17种单倍型仅在哈尔滨种群中出现。褐家鼠湛江种群D-loop区的核苷酸多态性为0.005，有27个变异位点，单倍型多态性为0.695，褐家鼠哈尔滨种群D-loop区的核苷酸多态性比湛江种群略高，为0.008，有35个变异位点，单倍型多态性为 0.793。褐家鼠湛江种群和哈尔滨种群没有经历过暴发性的扩增。褐家鼠湛江、哈尔滨和湖北3个地理种群的D-loop序列之间发生了明显的遗传分化，其中湛江种群和哈尔滨种群之间的分化程度最高，遗传分化系数Fst为0.245。褐家鼠湛江种群和哈尔滨种群的单倍型数目和主单倍型频率都发生明显波动，推测主要原因可能是由于灭鼠剂的大量使用或其他灭鼠活动导致种群出现瓶颈或更替的现象。
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| 关 键 词： | 褐家鼠 D-loop区 单倍型 谱系地理 遗传多样性 |
| 收稿时间： | 2020/3/11 0:00:00 |
Characterization of population structure and its annual variation in Norway rat Rattus norvegicus in Zhanjiang and Harbin cities in China |
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| Chen Yan,Ma Xiaohui,Wang Dawei,Li Ning,Yao Dandan,Feng Zhiyong,Liu Xiaohui,Song Ying.Characterization of population structure and its annual variation in Norway rat Rattus norvegicus in Zhanjiang and Harbin cities in China[J].Acta Phytophylacica Sinica,2021,48(2):449-457. |
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| Authors: | Chen Yan Ma Xiaohui Wang Dawei Li Ning Yao Dandan Feng Zhiyong Liu Xiaohui Song Ying |
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| Institution: | State Key Laboratory for Biology of Plant Diseases and Insect Pest, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China |
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| Abstract: | In order to understand the population genetic structure of the Norway rat Rattus norvegicus and its annual variation in China, the D-loop sequences of the rats from Zhanjiang and Harbin cities were sequenced. Combined with the other D-loop sequences of rats from China and other countries, the genetic diversity and phylogenetic network between Zhanjiang and Harbin populations, particularly the annual variation of D-loop haplotypes and their frequencies from 2008 to 2015 were analyzed. The results showed that there were 32 different haplotypes in Zhanjiang and Harbin populations, of which 11 were shared between the two populations, four and 17 were unique in Zhanjiang and Harbin populations, respectively. The D-loop nucleotide diversity in Zhanjiang population was 0.005 with 27 variable sites, and the haplotype diversity was 0.695; the D-loop nucleotide diversity in Harbin population was 0.008 with 35 variable sites, which was slightly higher than that of Zhanjiang population with a haplotype diversity of 0.793. No signal of demographic expansion was detected in Zhanjiang and Harbin populations. The population genetic differentiation (Fst) was significant among rat populations in Zhanjiang City, Harbin City and Hubei Province, and the highest Fst (0.245) was found between the former two populations. Analysis of the annual populations in both areas suggested significantly fluctuated D-loop haplotype numbers and frequencies among them, indicating population bottleneck or replacement occurring in both populations owing to the extensive use of rodenticides or other rodent control activities. |
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| Keywords: | Norway rat D-loop region haplotype phylogeography genetic diversity |
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