| 用酶免疫测定法检测鱼害粘球菌的试验 |
| |
| 引用本文： | 陈月英,黄惟灏,董济海.用酶免疫测定法检测鱼害粘球菌的试验[J].水产学报,1981,5(1):75-80. |
| |
| 作者姓名： | 陈月英 黄惟灏 董济海 |
| |
| 作者单位： | 浙江省淡水水产研究所
(陈月英，黄惟灏)，浙江省淡水水产研究所(董济海) |
| |
| 摘 要： | 本文介绍了不标记抗体酶免疫测定法检测鱼害粘球菌烂鳃病的试验结果。试验表明，鱼害粘球菌不同菌株的菌体都能与其各特异抗血清交叉反应，用酶标记显示棕色，获得阳性反应，其细长弯曲的菌体形态在光学显微镜下清晰可见。 用正常兔血清或含吐温20O．1M，pH7．4的磷酸缓冲液或吸收血清，或用Cg25和Cg27菌株代替鱼害粘球菌的对照组，酶标记都呈阴性反应，菌体不呈棕色，在光学显微镜下观察有明显差别。以健康草鱼鳃上的粘液作对照试验，粘液本身呈淡黄色，只有红血球呈深棕色。这种检测法对粘细菌病的流行病学调查或用于诊断鱼病有一定实际意义。这一检测技术对其他鱼类的病毒病的病原定位、传染源、传染途径的探索，以及对细菌性鱼病或寄生虫鱼病的研究也可能是适用的。
|
| 关 键 词： | 酶免疫测定法 血清 诊断 棕色 粘液 性反应 对照组 鱼害粘球菌 细菌性鱼病 试验 |
EXPERIMENT ON THE EXAMINATION OF MYXOCOCCUS PISCICOLA BY THE ENZYME--IMMUNOASSAY METHOD |
| |
| Chen Yueying,Huang Weihao and Dong Jihai.EXPERIMENT ON THE EXAMINATION OF MYXOCOCCUS PISCICOLA BY THE ENZYME--IMMUNOASSAY METHOD[J].Journal of Fisheries of China,1981,5(1):75-80. |
| |
| Authors: | Chen Yueying Huang Weihao and Dong Jihai |
| |
| Institution: | Freshwater Fisheries Reaeareh Institute of Zhanjiang;Freshwater Fisheries Reaeareh Institute of Zhanjiang;Freshwater Fisheries Reaeareh Institute of Zhanjiang |
| |
| Abstract: | By using unlabeled enzyme-immunoassay method to examine the gill disease cau-sed by the bacteria Myxococcus pisicola good results were obtained. The experimentsmanifest that the bacteria from different strains gave cross reaction to specific anti-serum, and the enzyme-immunoassay shows positive reaction. A brown color appeared,and the curved slender bacteria could easily be observed under the microscope. In thecross set, including the nomal rabbit serum, PBS-T, the absorbed serum, the Cg25strains or Cg27 strains were used to substitute the bacteria for checking. The results all gave negative reaction, and no brown color appeared. Evident differences could bedistinguished under microscope. If the mucus is taken from the gills of the healthygrass carp for checking, it appears light yellow in color, only the red blood corpusclesgiving dark brown color. This method of examination has certain practical uses for the survey of prevailingmucous bacteria disease and for the dignosis on other fish diseases. It may be also usefulin localizing pathogen of virulent diseases, in tracing the source and the path of infec-tion, and in studying bacterial and parasitological diseases of fishes. |
| |
| Keywords: | |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《水产学报》浏览原始摘要信息 |
| 点击此处可从《水产学报》下载免费的PDF全文 |
