| 致鹅卵黄性腹膜炎大肠杆菌的分离与16SrDNA的鉴定 |
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| 引用本文： | 钱琨,张永志,金文杰,邵红霞,秦爱建.致鹅卵黄性腹膜炎大肠杆菌的分离与16SrDNA的鉴定[J].中国家禽,2011,33(14). |
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| 作者姓名： | 钱琨 张永志 金文杰 邵红霞 秦爱建 |
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| 作者单位： | 扬州大学兽医学院,江苏省动物预防医学重点实验室,禽类预防医学省部共建教育部重点实验室,江苏扬州 225009 |
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| 基金项目： | 江苏省高校自然科学重大基础研究项目(07KJA23021) |
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| 摘 要： | 从鹅卵黄性腹膜炎临床病例中采集样品,分离病原进行一系列生化鉴定,共分离得到7株致鹅卵黄性腹膜炎大肠杆菌,编号为E0238、E0239、E0240、E0453、E0454、E0241、E0245。O血清型鉴定分属于O2、O21、O37、O1、O24、O24、O148。利用16SrDNA扩增试剂盒对随机选取的3株细菌进行PCR扩增,并将PCR产物序列与GenBank中的E.coli16SrDNA进行序列比对,结果显示分离细菌与大肠杆菌的同源性达99%以上。
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| 关 键 词： | 鹅卵黄性腹膜炎 大肠杆菌 16SrDNA 分离鉴定 |
Isolation and 16 S rDNA Identification of E.coli Strains from Geese Infected with Yolk-sac Peritonitis |
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| QIAN Kun,ZHANG Yongzhi,JIN Wenjie,SHAO Hongxia,QIN Aijian.Isolation and 16 S rDNA Identification of E.coli Strains from Geese Infected with Yolk-sac Peritonitis[J].China Poultry,2011,33(14). |
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| Authors: | QIAN Kun ZHANG Yongzhi JIN Wenjie SHAO Hongxia QIN Aijian |
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| Institution: | QIAN Kun,ZHANG Yongzhi,JIN Wenjie,SHAO Hongxia,QIN Aijian**(Key Lab for Avian Preventive Medicine of Ministry of Education & Key Lab of Jiangsu Preventive Veterinary Medicine,College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009) |
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| Abstract: | Samples from dead geese infected with yolk-sac peritonitis were collected from the veterinary stations.Seven bacteria strains were isolated and identified by biochemistry tests,which named as E0238,E0239,E0240,E0453,E0454,E0241 and E0245.The O antigen test results showed that O serotypes of these strains were O2,O21,O37,O1,O24,O24 and O148 respectively.Three random selected strains were detected by polymerase chain reaction(PCR) and compared with 16 S rDNA sequence of Escherichina coli(E.coli) from GenBank.... |
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| Keywords: | geese yolk-sac peritonitis E coli 16 S rDNA isolation and identification |
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