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Isozyme polymorphism of Rosa spp. and cultivar identification
Authors:Cédric Grossi  Olivier Raymond  Maurice Jay
Institution:(1) Laboratoire de Biologie Micromoléculaire et Phytochimie, Université Claude Bernard Lyon I, 43, bd du 11 novembre 1918, 69622 Villeurbanne Cedex, France
Abstract:In order to study the polymorphism of the enzyme systems of rose cultivars, experimental conditions were determined to simultaneously extract three systems: esterase (EST), leucine aminopeptidase (LAP) and superoxide dismutase (SOD) which gave up to 9, 7 and 9 bands, respectively. The influence of environmental conditions on the polymorphism level was taken into account. Finally, the computation of the Jaccard distances from the distribution of the isozyme bands led to the improvement of the identification process within an Ancient Rose cultivar collection. This revised version was published online in July 2006 with corrections to the Cover Date.
Keywords:cluster analysis  cultivar identification  electrophoresis  isozyme  Rosa spp  stability
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