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| 新疆阿尔泰地区白杨派3个树种半同胞家系子代遗传多样性分析 | |
| 引用本文： | 郑书星,张建国,段爱国,何彩云,保尔江,王健.新疆阿尔泰地区白杨派3个树种半同胞家系子代遗传多样性分析[J].林业科学研究,2013,26(3):366-372. |
| 作者姓名： | 郑书星 张建国 段爱国 何彩云 保尔江 王健 |
| 作者单位： | 1. 中园林业科学研究院林业研究所,林木遗传育种国家重点实验室,国家林业局林木培育重点实验室,北京100091;中国林业科学研究院资源昆虫研究所,云南昆明650224 2. 中园林业科学研究院林业研究所,林木遗传育种国家重点实验室,国家林业局林木培育重点实验室,北京100091 3. 阿勒泰地区林业科学研究所,新疆阿勒泰,836500 |
| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金项目"额尔齐斯河天然杨树种群遗传多样性研究"(201004035) |
| 摘 要： | 利用SSR分子标记技术,对采自新疆额尔齐斯河流域阿尔泰市哈巴河及北屯地区的白杨派3个树种银白杨、银灰杨、欧洲山杨的半同胞家系子代进行遗传多样性分析.结果表明:筛选12对SSR引物在90个样本中共检测到58个等位基因(A)、112种基因型,多态位点百分率是100％,Nei遗传多样性指数(h)平均为0.648 5,Shannon多样性指数平均为1.234.基因分化系数(Fst)为0.337 l,即在总的遗传变异中有33.71％的变异来自于不同树种之间,绝大部分存在于子代个体间.白杨派内种间分化程度较高,种间遗传距离为0.3863～1.869;银灰杨的遗传变异最大,而银白杨的遗传变异最小. |
| 关 键 词： | 白杨派 SSR 遗传多样性 半同胞家系 |
| 收稿时间： | 6/9/2009 12:00:00 AM |
| 修稿时间： | 2013/3/10 0:00:00 |
The Genetic Diversity of Half-sib Family Progenies of Populus (Section Leuce) in Altai of Xinjiang |
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| ZHENG Shu-xing,ZHANG Jian-guo,DUAN Ai-guo,HE Cai-yun,BAO Er-jiang and WANG Jian.The Genetic Diversity of Half-sib Family Progenies of Populus (Section Leuce) in Altai of Xinjiang[J].Forest Research,2013,26(3):366-372. | |
| Authors: | ZHENG Shu-xing ZHANG Jian-guo DUAN Ai-guo HE Cai-yun BAO Er-jiang and WANG Jian |
| Institution: | Research Institute of Forestry, Chinese Academy of Forestry, State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;Research Institute of Resource Insects, Chinese Academy of Forestry, Kunming 650224, Yunnan, China;;Research Institute of Forestry, Chinese Academy of Forestry, State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;;Research Institute of Forestry, Chinese Academy of Forestry, State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;;Research Institute of Forestry, Chinese Academy of Forestry, State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;;Aletai Research Institute of Forestry, Aletai 836500, Xinjiang, China;Aletai Research Institute of Forestry, Aletai 836500, Xinjiang, China |
| Abstract: | Ninety progenies representing three half-sib families of Leuce (Populus alba L., Populus canescens (Ait.) Smith, and Populus tremula L.) were analyzed by using microsatellite to study the genetic diversity. 58 alleles and 112 genotypes were identified based on 12 SSR primers. The percentage of polymorphic loci, the Nei's genetic diversity index and Shannon information index were 100%, 0.648 5 and 1.234. The coefficient of gene differentiation was 0.337 1, which indicated 66.29% of genetic variation resided within the progenies of species. The results from cluster analysis showed that there was abundant genetic variation among the three Leuce species, the genetic distance among species ranged from 0.386 3 to 1.869 and Populus canescens had the most genetic variation. |
| Keywords: | Leuce SSR genetic diversity half-sib families |
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