| 仔猪致泻性大肠杆菌毒力因子多重PCR检测方法的建立与应用 |
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| 引用本文： | 毕斓婷,冀亚路,袭恒豫,张昊,陈椿桦,姜秋杰,付殿国,牛伟,孙长江,冯新,吕伟,顾敬敏,韩文瑜.仔猪致泻性大肠杆菌毒力因子多重PCR检测方法的建立与应用[J].中国兽医学报,2022(2):250-255. |
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| 作者姓名： | 毕斓婷 冀亚路 袭恒豫 张昊 陈椿桦 姜秋杰 付殿国 牛伟 孙长江 冯新 吕伟 顾敬敏 韩文瑜 |
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| 作者单位： | 1.吉林大学动物医学学院;2.吉林省动物疫病预防控制中心;3.吉林农业科技学院动物科技学院;4.吉林省公主岭市鑫源木业 |
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| 基金项目： | 国家自然科学基金资助项目(U19A2038,32072824,31872505)。 |
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| 摘 要： | 本研究以致泻性大肠杆菌(DEC)的4种毒力因子EAST1、LT、STa和STb为模板设计特异性引物并优化各项反应条件,以建立仔猪DEC的多重PCR检测方法.该方法具有较好的特异性,只对DEC产生特异性条带,对非DEC及其他病菌则呈阴性.利用所建立的多重PCR方法对吉林省各猪场采集的样本进行检测,结果表明,在测定的猪场中...
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| 关 键 词： | 致泻性大肠杆菌 毒力因子 多重PCR |
Establishment of multiplex PCR detection method for diarrhea-causing Escherichia coli virulence factor in piglets in Jilin Province |
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| BI Lanting,JI Yalu,XI Hengyu,ZHANG Hao,CHEN Chunhua,JIANG Qiujie,FU Dianguo,NIU Wei,SUN Changjiang,FENG Xin,LYU Wei,GU Jingmin,HAN Wenyu.Establishment of multiplex PCR detection method for diarrhea-causing Escherichia coli virulence factor in piglets in Jilin Province[J].Chinese Journal of Veterinary Science,2022(2):250-255. |
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| Authors: | BI Lanting JI Yalu XI Hengyu ZHANG Hao CHEN Chunhua JIANG Qiujie FU Dianguo NIU Wei SUN Changjiang FENG Xin LYU Wei GU Jingmin HAN Wenyu |
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| Institution: | (College of Veterinary Medicine,Jilin University,Changchun 130062,China;Jilin Province Animal Dis-ease Prevention and Control Center,Changchun 130062,China;College of Animal Science and Technology,Jilin Agricultural Science and Technology University,Jilin City,Jilin 132301,China;Gongzhuling Xinyuan Animal Husbandry Company,Gongzhuling,Jilin 136100) |
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| Abstract: | In this study, four virulence factors of diarrhea-causing Escherichia coli(DEC),EAST1,LT,STa and STb were used as templates to design specific primers and optimize various reaction conditions to establish a multiplex PCR detection method for piglet DEC. This method has good specificity and only produces specific bands for DEC,but is negative for non-DEC and other pathogens.The established multiplex PCR method was used to detect samples collected from pig farms in Jilin Province.The results showed that E.coli infections caused by positive strains carrying EAST1 virulence genes were widespread in the tested pig farms, and they carried STb virulence genes.Strains were followed by E.coli infections caused by positive strains carrying STa and LT virulence genes.Compared with the above two virulence factors, the proportion of the tested pig farms was slightly lower.In summary, the establishment of this method provides a new method for the rapid detection and molecular epidemiological investigation of common virulence factors in DEC. |
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| Keywords: | diarrhea-causing Escherichia coli(DEC) virulence factor multiplex PCR |
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