| 2种共轭亚油酸异构体对小鼠肌内脂肪沉积和肌肉线粒体代谢的影响 |
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| 引用本文： | 段晨,尹聪,李凡,张枫琳,马泽炜,凌明发,江青艳,束刚,王松波.2种共轭亚油酸异构体对小鼠肌内脂肪沉积和肌肉线粒体代谢的影响[J].中国畜牧杂志,2022(1):166-172. |
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| 作者姓名： | 段晨 尹聪 李凡 张枫琳 马泽炜 凌明发 江青艳 束刚 王松波 |
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| 作者单位： | 1.华南农业大学动物科学学院广东省动物营养调控重点实验室 |
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| 基金项目： | 国家自然科学基金(31972636,31790411)。 |
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| 摘 要： | 试验旨在研究不同共轭亚油酸异构体对小鼠肌内脂肪沉积和肌肉线粒体代谢的影响.将成长到5周龄的雄性小鼠按体重分成3组(每组8只),适应1周后分别饲喂不添加、添加2％的顺9反11共轭亚油酸(c9,t11-CLA)和添加2％的反10顺12共轭亚油酸(t10,c12-CLA)的日粮,正试期1个月,监测其体重和体组成的变化,饲养试...
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| 关 键 词： | 小鼠 共轭亚油酸 肌内脂肪 线粒体生物合成 氧化代谢 |
Effects of Two Different Conjugated Linoleic Acid Isomers on Intramuscular Fat Deposition and Muscle Mitochondrial Metabolism in Mice |
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| DUAN Chen,YIN Cong,LI Fan,ZHANG Fenglin,MA Zewei,LING Mingfa,JIANG Qingyan,SHU Gang,WANG Songbo.Effects of Two Different Conjugated Linoleic Acid Isomers on Intramuscular Fat Deposition and Muscle Mitochondrial Metabolism in Mice[J].Chinese Journal of Animal Science,2022(1):166-172. |
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| Authors: | DUAN Chen YIN Cong LI Fan ZHANG Fenglin MA Zewei LING Mingfa JIANG Qingyan SHU Gang WANG Songbo |
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| Institution: | ,Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science,South China Agricultural University |
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| Abstract: | This study was conducted to investigate the effects of different conjugated linoleic acid isomers(c9, t11-CLA, t10,c12-CLA) on intramuscular fat deposition and muscle mitochondrial metabolism in mice. Male mice at the age of 5 weeks were divided into 3 groups(8 mice per group) according to the body weight. After a week of adaptation, the control group was fed control diets while other two groups were fed diets supplemented with 2% c9,t11-CLA and 2% t10,c12-CLA respectively.The trial lasted for one month, and changes in body weight and body composition of mice were monitored. Serum samples were collected after slaughter to detect the contents of triglycerides and free fatty acids. Meanwhile, muscles were collected to detect the triglyceride content. Oil red O staining was used to directly detect the changes of intramuscular fat content.Total RNA was extracted from the gastrocnemius muscle, and changes of genes related to fatty acid transport, mitochondrial synthesis and oxidative metabolism were detected by fluorescence quantitative PCR. The results showed that dietary supplementation of c9, t11 and t10, c12 conjugated linoleic acid isomers significantly reduced body weight and body gain,decreased body fat proportion, increased muscle proportion, and decreased serum triglyceride content in mice. Compared with c9, t11 conjugated linoleic acid isomer, the supplementation of t10, c12 conjugated linoleic acid isomer significantly increased free fatty acid content in serum, triglyceride and intramuscular fat content in muscle, and increased the mRNA expression of fatty acid transporters, mitochondrial biosynthesis and oxidative metabolism genes in muscle. These results above indicated that t10, c12 conjugated linoleic acid plays a more significant role in intramuscular fat deposition, mitochondria biogenesis and oxidative metabolism in muscle. |
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| Keywords: | Mice Conjugated linoleic acid Intramuscular fat Mitochondrial biogenesis Oxidative metabolism |
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