Biosynthesis of monoterpenes and norisoprenoids in raspberry fruits (Rubus idaeus L.): the role of cytosolic mevalonate and plastidial methylerythritol phosphate pathway |
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| Authors: | Hampel Daniela Swatski Anna Mosandl Armin Wüst Matthias |
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| Institution: | Institut für Lebensmittelchemie, Johann Wolfgang Goethe-Universit?t, Max-von-Laue-Strasse 9, D-60438 Frankfurt/Main, Germany. |
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| Abstract: | The biosynthesis of the monoterpenes (-)-alpha-pinene, linalool, and the norisoprenoids alpha- and beta-ionone in raspberry fruits (rubus idaeus L.) was investigated by in vivo feeding experiments with 5,5-(2)H2]-mevalonic acid lactone and 5,5-(2)H2]-1-deoxy-D-xylulose. The volatile compounds were extracted by stirbar sorptive extraction and analyzed using thermal desorption-multidimensional gas chromatography-mass spectrometry (TD-enantio-MDGC-MS). The feeding experiments demonstrate that (-)-alpha-pinene and (S)-linalool are exclusively synthesized via the cytosolic mevalonic acid pathway. In contrast, (2)H-labeled (R)-(E)-alpha-ionone and (2)H-labeled (E)-beta-ionone are detectable after application of d2-1-deoxy-D-xylulose and d2-mevalonic acid lactone, respectively. However, (R)-linalool reveals no incorporation of either one of the fed precursors, even though this enantiomer is detectable in the fruit tissue. |
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