| 黄粉虫几丁质/壳聚糖提取工艺的研究 |
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| 引用本文： | 范文艳,王丽艳.黄粉虫几丁质/壳聚糖提取工艺的研究[J].黑龙江农业科学,2010(4):122-125,147. |
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| 作者姓名： | 范文艳 王丽艳 |
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| 作者单位： | 黑龙江八一农垦大学,农学院,黑龙江,大庆,163319 |
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| 摘 要： | 通过正交试验对黄粉虫几丁质/壳聚糖的提取工艺进行了优化。结果表明:盐酸浓度、脱乙酰基氢氧化钠浓度和盐酸处理时间对壳聚糖提取有较大影响,其影响程度顺序为盐酸浓度氢氧化钠浓度盐酸处理时间。黄粉虫几丁质/壳聚糖的提取最佳工艺条件为10%盐酸室温浸泡2~6 h,7%氢氧化钠100℃煮2.5 h,50%氢氧化钠85℃水浴8 h。在最佳提取工艺下,壳聚糖的提取率达15%,壳聚糖游离氨基大于80%,灰分小于1%,水分4.91%~5.01%。
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| 关 键 词： | 黄粉虫 几丁质 壳聚糖 提取工艺 |
Study on Extraction Process of Chitin/Chitosan from Tenebrio molitor L. |
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| FAN Wen-yan,WANG Li-yan.Study on Extraction Process of Chitin/Chitosan from Tenebrio molitor L.[J].Heilongjiang Agricultural Science,2010(4):122-125,147. |
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| Authors: | FAN Wen-yan WANG Li-yan |
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| Institution: | (Agronomy College of Heilongjiang August First Land Reclamation University,Daqing,Heilongjiang 163319) |
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| Abstract: | The orthogonalty experiment was used to optimize the extraction conditions of chitin/chitosan from Tenebrio molitor L.. The results showed that HCl concentration, NaOH concentration in the process of deacetylation and HCl treating time significantly affect the extraction rate of chitin/chitosan, and the order of effect degree was as follows: HCl concentration＞NaOH concentration＞HCl treating time. The optimal extraction process were 10% HCl soaking for 2 h to 6 h at room temperature, 7% NaOH boiling for 2.5 h at 100 ℃ and treated with 50% NaOH for 8 h at 85℃. Under these conditions, the extracting rate of chitin/chitosan was 15%. The dissociative amidogen of chitin/chitosan was more than 80%. The ash content of chitin/chitosan less than 1%. The water content of chitin/chitosan was 4.91%～5.01%. |
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| Keywords: | Tenebrio molitor L chintin/chitosan extraction process |
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