| 魔芋花粉的低温和超低温保存 |
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| 引用本文： | 张玉进,张兴国,刘佩瑛.魔芋花粉的低温和超低温保存[J].园艺学报,2000,27(2):139-142. |
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| 作者姓名： | 张玉进 张兴国 刘佩瑛 |
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| 作者单位： | 1. 华南师范大学生物系,广州,510631
2. 西南农业大学园艺系,重庆,400716 |
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| 基金项目： | 国家自然科学基金! (30 6 70 5 13)，博士学科点基金! (95 0 6 0 5 ) |
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| 摘 要： | 利用干燥法和玻璃化法，魔芋花粉能够在4℃下保存1个月，－20℃下保存6个月；魔芋花粉经液氮超低温（－196℃）保存后，有明显的“冷刺激”现象，即保存花粉比新鲜花粉有更高的离体萌发率。
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| 关 键 词： | 魔芋 花粉 低温保存 超低温保存 冷刺激现象 |
| 修稿时间： | 1999-03-18 |
Conservation of Amorphophallus Pollen at Low Temperature and in Liquid Nitrogen |
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| Zhang Yujin,Zhang Xingguo,Liu Peiying.Conservation of Amorphophallus Pollen at Low Temperature and in Liquid Nitrogen[J].Acta Horticulturae Sinica,2000,27(2):139-142. |
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| Authors: | Zhang Yujin Zhang Xingguo Liu Peiying |
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| Institution: | 1. Department of Molecular Biology, Infectious Disease Center, the 458 th Hospital of PLA, Guangzhou 510602; 2. Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, China |
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| Abstract: | AIM: To observe the effect of cardiomyopeptidin (CMP) on incidence rate of ventricular tachycardia(VT) and ventricular fibrillation(VF)and the influence of CMP on the inward calcium current and potassium current. METHODS: The ischemia-reperfusion model were developed in rat heart in vivo. The influence of CMP on the inward calcium current(ICa) and its current-voltage relationship,the inward rectifier and delayed rectifier potassium current were recorded by patch-clamp techniques in guinea pig ventricular myocytes. RESULTS: The results showed that CMP could decrease the incidence rate of VT and VF, and could inhibited the inward calcium current of myocardial cells in certain concentration range and in a voltage-dependent manner. But CMP had no effect on both of inward rectifier and delayed rectifier potassium current. CONCLUSION: CMP could decrease the incidence rate of serious ventricular arrhythmia in rat heart injured by ischemia-reperfusion,this may be related to its decreasing ICa in ventricular myocytes. |
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| Keywords: | Amorphophallus Pollen Storage Cryopreservation |
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