Antibody Responses in Sheep Vaccinated against Staphylococcus aureus Mastitis: A Comparison of Two Experimental Vaccines Containing Different Adjuvants |
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| Authors: | Tollersrud T Nørstebø PE Engvik JP Andersen SR Reitan LJ Lund A |
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| Institution: | (1) Department of Immunoprophylaxis, National Veterinary Institute, P.B. 8156, 0033 Oslo Dep., Norway;(2) Norwegian School of Veterinary Science, Oslo, Norway;(3) Noragric, Agricultural University of Norway, Ås, Norway |
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| Abstract: | A double-blind, placebo-controlled study was conducted to compare two vaccines using different adjuvants with regard to their ability to stimulate antibody production against the  - and  -toxins and the exopolysaccharide of Staphylococcus aureus. The vaccines contained identical antigens, consisting of inactivated whole bacteria of two strains of S. aureus in addition to - and -toxoid. One vaccine contained mineral oil, while the other used a water-soluble acrylic acid polymer resin (Carbopol) as adjuvant. Saline served as the placebo. One hundred and forty ewes were vaccinated twice before lambing, by subcutaneous injection with vaccine or placebo in the region of the supramammary lymph node, and were observed and sampled over a period of 6 months. The vaccine containing mineral oil as adjuvant induced significantly greater immune responses to the - and -toxins than did the vaccine containing Carbopol. The latter vaccine induced higher levels of antibodies to exopolysaccharide. The degree of local adverse reactions did not differ between the two groups. The results indicate differences between the oil-adjuvanted and Carbopol-adjuvanted vaccines with regard to their ability to stimulate antibody production against S. aureus protein antigens in sheep. |
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| Keywords: | acrylic acid polymer resin adjuvant immune response mineral oil sheep Staphylococcus aureus toxin vaccine |
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