| 橘小实蝇不育雄虫与野生雄虫间遗传分化 |
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| 引用本文： | 季清娥,杨慧,陈家骅,胡美玲.橘小实蝇不育雄虫与野生雄虫间遗传分化[J].核农学报,2009,23(2):349-353. |
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| 作者姓名： | 季清娥 杨慧 陈家骅 胡美玲 |
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| 作者单位： | 1. 福建农林大学植物保护学院,福建,福州,350002
2. 宁德出入境检验检疫局,福建,宁德,352100 |
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| 基金项目： | 福建省重大科技专项项目，福建省发改委专项基金，国际原子能机构项目 |
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| 摘 要： | 对橘小实蝇不育雄虫和福建7个不同地区的野生雄虫共37个个体的线粒体细胞色素氧化酶I（mtDNA COI）基因进行了部分序列测定。在获得的650bp序列中，共有72个变异位点、32个单倍型，其中共享单倍型3种；对所有单倍型聚类分析发现，单倍型在系统树中的分布散乱，没有显示出明显的地理分布族群；结合遗传变异指数（Fst）和净遗传距离（Da）分析认为橘小实蝇不育雄虫与野生虫源间、不同来源的野生虫源间已存有一定程度的遗传分化，但分化程度较低。研究认为应用昆虫不育技术防治橘小实蝇，在饲养过程中需针对不同防治区引进野生虫源复壮，以确保不育雄虫和野生虫源相匹配，提高防治效果。
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| 关 键 词： | 橘小实蝇 不育雄虫 野生雄虫 线粒体DNA 细胞色素氧化酶Ⅰ基因 遗传分化 |
| 收稿时间： | 2008-07-22 |
Genetic Differentiation Between Sterile Male and Wild Male Bactrocera dorsalis (Hendel) |
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| JI Qing-e,YANG Hui,CHEN Jia-hua,Hu Mei-ling.Genetic Differentiation Between Sterile Male and Wild Male Bactrocera dorsalis (Hendel)[J].Acta Agriculturae Nucleatae Sinica,2009,23(2):349-353. |
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| Authors: | JI Qing-e YANG Hui CHEN Jia-hua Hu Mei-ling |
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| Institution: | 1.College of Plant Protection;Fujian Agriculture and Forestry University;Fuzhou;Fujian 350002;2.Ningde Entry-Exit Inspection and Quarantine Bureau;Ningde;Fujian 352100 |
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| Abstract: | Partial mitochondrial cytochrome oxidase I(mtDNA COI)region was sequenced from 37 individuals of sterile males and wild males of Bactrocera dorsalis(Hende1)from 7 different regions of Fujian Province.The 650bp sequence was obtained in which 72 variable sites and 32 haplotypes with 3 shared were identified.Phylogenetic trees of haplotypes were constructed by N-J method and the tree showed that all haplotypes did not accord with their regions.At the same time,the genetic differentiation index(Fst)and genetic ... |
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| Keywords: | Bactrocera dorsalis (Hendel) sterile male wild male mtDNA cytochrome oxidaseⅠ genetic differentiation |
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