| 重组杆状病毒表达的EIAV Env蛋白与含有env基因重组痘苗病毒的联合免疫 |
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| 引用本文： | 代春铭,张晓燕,张然然,邵一鸣,沈荣显.重组杆状病毒表达的EIAV Env蛋白与含有env基因重组痘苗病毒的联合免疫[J].畜牧市场,2009(2):12-16. |
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| 作者姓名： | 代春铭 张晓燕 张然然 邵一鸣 沈荣显 |
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| 作者单位： | 代春铭,DAI Chun-ming(中国疾病预防控制中心性病艾滋病预防与控制中心病毒免疫室,北京,100050;首都医科大学免疫学系,北京,100054);张晓燕,张然然,ZHANG Xiao-yan,ZHANG Ran-ran(中国疾病预防控制中心性病艾滋病预防与控制中心病毒免疫室,北京,100050);邵一鸣,SHAO Yi-ming(中国疾病预防控制中心性病艾滋病预防与控制中心病毒免疫室,北京,100050;中国农业科学院哈尔滨兽医研究所疫病室,黑龙江哈尔滨,150001);沈荣显,SHEN Rong-xian(中国农业科学院哈尔滨兽医研究所疫病室,黑龙江哈尔滨,150001)
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| 摘 要： | 目的：构建新型的马传染性贫血病毒（EIAV）的候选疫苗。方法：利用BAC-To-BAC杆状病毒表达系统，将中国马传贫驴白细胞弱毒疫苗（EIAVDLV）及其亲本株（EIAVLN）env基因导入到杆状病毒基因组中。转染昆虫细胞后，得到的重组病毒用SDS-PAGE和Western blot检测表达产物。以本实验室构建的含有EIAVEnv基因的重组痘苗病毒，单独或与重组杆状病毒表达的EIAVEnv蛋白联合免疫小鼠。结果：构建的重组杆状病毒能正确表达全长Env蛋白。与单独免疫组相比，联合免疫组免疫应答显著增强，其中中和抗体的滴度提高5—9倍。结论：含有EIAVEnv基因的重组痘苗病毒与Env蛋白抗原联合务埔．能够诱导高滴座的中和抗体．
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| 关 键 词： | EIAV Env蛋白 重组杆状病毒 重组痘苗病毒 免疫 |
Combination immunization with EIAV Env protein expressed by recombinant baeuiovirus and recombinant vaccinia virus containing env gene |
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| DAI Chun-ming,ZHANG Xiao-yan,ZHANG Ran-ran,SHAO Yi-ming,SHEN Rong-xian.Combination immunization with EIAV Env protein expressed by recombinant baeuiovirus and recombinant vaccinia virus containing env gene[J].stockbreeding market,2009(2):12-16. |
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| Authors: | DAI Chun-ming ZHANG Xiao-yan ZHANG Ran-ran SHAO Yi-ming SHEN Rong-xian |
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| Institution: | 1.Division of Research on Virology and Immunology , National Center for AIDS/ STD Control and Prevention CDC , Beijing 100050 ; 2.Department of Immunology , Capital University of Medical Sciences , Beijing 100054 ; 3.Division of Research on EIAV , naerbin Veterinary Research Institute , Uaerbin 150001 , China) |
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| Abstract: | AIM：To develop a novel vaccine candidate of Equine infectious anemia virus （EIAV）. METHODS ： env genes of EIAV Chinese donkey leukocyte attenuated strain （EIAV DLV） and its parental virus strain （EIAV LN） were expressed using the BAC-To-BAC system, and Env proteins were confirmed by SDS-PAGE and Western blot. BALB/ c mice were immunized with recombinant vaccinia viruses containing env genes of EIAV alone or boosted with Env proteins expressed by recombinant baculovirus. Both protective humoral and cellular immune responses were detected. RESULTS ： Recombinant baculovirus could express complete Env proteins and the titer of neutralizing antibody in the prime2boo st group was found to be 5 to 9 folds higher than that in the rVV group. CONCLUSION ： Combination of protein antigen and recombinant vaccinia virus could induce high titer of neutralizing antibody against EIAV. This work facilitates development of a novel genetically engineered vaccine of EIAV as a model for fu2 ture development of an HIV vaccine. |
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| Keywords: | EIAV Env protein recombinant baculo virus recombinant vaccinia virus Immune response |
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