In vivo and in vitro effect of recombinant salmon growth hormone treatment on IGF-I and IGFBPs in yellowtail <Emphasis Type="Italic">Seriola quinqueradiata</Emphasis> |
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| Authors: | Fiona L Pedroso Haruhisa Fukada Toshiro Masumoto |
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| Institution: | (1) United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Ehime, Japan;(2) Faculty of Agriculture, Kochi University, 200 Monobe, Nankoku, Kochi 783-8502, Japan |
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| Abstract: | The role of growth hormone (GH) in regulating hepatic mRNA expression of insulin-like growth factor-I (IGF-I) and IGF binding
proteins (IGFBPs) in yellowtail Seriola quinqueradiata was examined using in vivo and in vitro assays. Yellowtail hepatic IGF-I, IGFBP-1, IGFBP-2, IGFBP-3, and IGFBP-5 mRNAs were
measured by real-time quantitative RT-PCR. Intraperitoneal injection of recombinant GH of chum salmon Oncorhynchus keta (rsGH) at a dose of 1 μg/g body weight resulted in a significant increases in hepatic IGF-I, IGFBP-3, and IGFBP-5 mRNA levels,
whereas significant reductions in hepatic IGFBP-1 and IGFBP-2 mRNA levels were observed. For in vitro assays, liver slices
were incubated with rsGH at different concentrations (doses: 0, 1, 10, 100, 500, and 1,000 ng/ml). Liver slices incubated
with 100 ng/ml rsGH elicited a significant increase in IGF-I mRNA level. Similarly, a slight increase in IGFBP-3 and IGFBP-5
mRNA levels were also observed in liver explants incubated with rsGH. In contrast, a significant decline in IGFBP-1 mRNA levels
was observed in liver slices incubated with 1,000 ng/ml rsGH. A slight decline in the level of IGFBP-2 mRNA was noted in liver
explants with rsGH treatment. This study demonstrates the modulating effect of GH on the IGF system. |
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| Keywords: | Growth hormone IGF-I IGF-binding proteins IGF system Yellowtail |
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