| 山羊瘤胃微生物宏基因组文库中一个新型纤维素酶 基因的克隆与表达 |
| |
| 引用本文： | 姜海琴,程建波,王力生,李吕木,范彩云.山羊瘤胃微生物宏基因组文库中一个新型纤维素酶 基因的克隆与表达[J].安徽农业大学学报,2015,42(2):209-212. |
| |
| 作者姓名： | 姜海琴 程建波 王力生 李吕木 范彩云 |
| |
| 作者单位： | 安徽农业大学动物科技学院,合肥,230036;安徽农业大学动物科技学院,合肥,230036;安徽农业大学动物科技学院,合肥,230036;安徽农业大学动物科技学院,合肥,230036;安徽农业大学动物科技学院,合肥,230036 |
| |
| 基金项目： | 安徽省自然科学基金项目(090411019), 安徽省教育厅自然科学基金（KJ2013A115）和高校博士点基金项目(20133418120002)共同资助。 |
| |
| 摘 要： | 通过功能筛选方法,从安徽白山羊瘤胃微生物宏基因组文库中筛选得到了6个纤维素酶的阳性克隆。对其中的1个阳性克隆进一步进行亚克隆和序列分析,获得一个新型纤维素酶基因开放阅读框,通过BLAST对基因全序列进行分析比较,发现其与来自Bacteroides cellulosilyticus DSM 14838的hypothetical protein具有51%的序列相似性和65%的同源性。并以p ET28a(+)为载体、Escherichia coli BL21(DE3)为宿主菌,对新型酶基因进行高效重组表达,并对该酶表达条件进行优化。
|
| 关 键 词： | 瘤胃微生物 宏基因组 纤维素酶 |
| 收稿时间： | 2014/4/28 0:00:00 |
Cloning and expression of a new cellulase gene from the metagenomic library of the goat rumen |
| |
| JIANG Haiqin,CHENG Jianbo,WANG Lisheng,LI Lvmu and FAN Caiyun.Cloning and expression of a new cellulase gene from the metagenomic library of the goat rumen[J].Journal of Anhui Agricultural University,2015,42(2):209-212. |
| |
| Authors: | JIANG Haiqin CHENG Jianbo WANG Lisheng LI Lvmu and FAN Caiyun |
| |
| Institution: | School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036 and School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036 |
| |
| Abstract: | Six positive clones with cellulase activities were isolated from a metagenomic library of the goat rumen using a functional screening method. One positive clone was subject to further subcloning and sequence analysis and a new cellulase gene open reading frame was obtained. After blast analysis, it was found that the cellulase gene showed 51% sequence similarity and 65% homology compared with the hypothetical protein of Bacteroides cellulosilyticus DSM 14838. Using pET28a (+) as a carrier, Escherichia coli BL21 (DE3) as a host strain, the new gene was recombined and expressed efficiently and the expression conditions of the newly-cloned gene were optimized. |
| |
| Keywords: | rumen microbes metagenomic cellulase |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《安徽农业大学学报》浏览原始摘要信息 |
| 点击此处可从《安徽农业大学学报》下载免费的PDF全文 |
|
