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Influence of orally administered CpG-ODNs on the humoral response to bovine serum albumin (BSA) in chickens |
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| Authors: | Ameiss K A El Attrache J Barri A McElroy A P Caldwell D J |
| Institution: | aPoultry Health Research Laboratory, Texas A&M University, College Station, TX, USA bDepartment of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA |
| Abstract: | Synthetic oligodeoxynucleotides containing CpG motifs (CpG-ODNs) have been reported to be effective mucosal adjuvants in mice when given orally. Studies on their effectiveness in chickens are currently very limited. This study investigated whether CpG-ODNs could adjuvant the immune response to BSA when given orally to a commercial line of SCWL chickens. In two experiments, performed over time, chickens were given selected concentrations of CpG-ODNs with BSA followed by 6 consecutive days of ad libitum access to drinking water containing 1.4 mg/ml BSA. Serum responses, and in some cases intestinal specific antibodies, were measured out to 33 days post-immunization. Birds receiving a single dose of CpG-ODN had consistently higher IgG, IgM, and IgA titers in the serum, dependent upon dose, and in specific areas of the intestine when compared to the non-immunized and BSA only groups. These findings suggest that a single oral CpG-ODN administration can accelerate the kinetics of antigen specific antibodies of all three isotypes in commercial-strain chickens immunized via the drinking water using common protein antigen. |
| Keywords: | CpG-ODN Chicken ELISA Immunomodulation Adjuvant BSA Antibodies Oral immunization |
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