| 不同条件对绒山羊体外培养初级毛囊生长的影响 |
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| 引用本文： | 李蘅,李金泉,曹贵方,张文广.不同条件对绒山羊体外培养初级毛囊生长的影响[J].安徽农业科学,2008,36(36). |
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| 作者姓名： | 李蘅 李金泉 曹贵方 张文广 |
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| 作者单位： | 1. 内蒙古农业大学生物工程学院,内蒙古呼和浩特,010018
2. 内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特,010018 |
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| 基金项目： | 国家自然科学基金地区重点项目，内蒙古自治区自然科学基金重大项目 |
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| 摘 要： | 目的]为阐述毛囊生物学特性和生长调控机制奠定基础。方法]在无菌条件下分离绒山羊初级毛囊并培养于无血清DMEM培养基和Williams E培养基中,观测毛囊的生长速度及形态学变化。结果]毛囊在无血清DMEM培养基中的生长速度是0.034 mm/d(前3d),在生长过程中毛囊的层次结构及形态可长时间保持不变;在无血清Williams E培养基中生长速度是0.077 mm/d(前3 d),明显加快。结论]绒山羊初级毛囊在2种培养基中生长速度差异显著(P<0.05),更适合在Williams E无血清培养基中生长。
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| 关 键 词： | 绒山羊 初级毛囊 体外培养 |
Effects of Different Culture Conditions in vitro on Growth of Cashmere Goat Primary Hair Follicles |
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| Abstract: | Objective] The aim of this study is to lay a foundation for illustrating the biological characteristics and growth regulation mechanism of hair follicles.Method] Cashmere goat primary hair follicles were separated under aseptic condition and cultured in serum-free DMEM and Williams E media respectively;subsequently,the growth rate and morphological changes were observed under the inverted microscope.Result] Hair follicles cultured in serum-free DMEM media showed a growth rate of 0.034 mm/d during the first 3 days,whose structure and morphological characteristics could maintian a stable status for a long time in the growth process.Hair follicles grew much faster in the serum-free Williams E media with a growth rate of 0.077 mm/d during the first 3 days.Conclusion] There were significant differences(P<0.05) between the growth rate of cashmere goat hair follicles in the two kinds of media.Serum-free Williams E medium was superior to serum-free DMEM medium. |
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| Keywords: | Cashmere goat Primary hair follicle Culture in vitro |
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