| 荞麦籽粒乙醇提取物抗氧化成分的高效液相色谱电喷雾质谱研究 |
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| 引用本文： | 姚亚平,田呈瑞,曹炜.荞麦籽粒乙醇提取物抗氧化成分的高效液相色谱电喷雾质谱研究[J].中国农业科学,2008,41(7):2092-2097. |
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| 作者姓名： | 姚亚平 田呈瑞 曹炜 |
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| 作者单位： | 陕西师范大学食品工程与营养科学学院 |
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| 摘 要： | 【目的】研究甜荞麦和苦荞麦籽粒乙醇提取物中的主要抗氧化成分。【方法】将荞麦籽粒乙醇提取物与二苯基苦基苯肼（DPPH）自由基反应，然后用高效液相色谱仪识别乙醇提取物中的抗氧化成分，并用电喷雾质谱检测器对抗氧化成分的结构信息进行表征，以确定荞麦籽粒乙醇提取物中的抗氧成分。【结果】苦荞麦和甜荞麦籽粒乙醇提取物对DPPH自由基均有清除作用；高效液相色谱分析表明，甜荞麦和苦荞麦乙醇提取物中均含有两种主要抗氧化峰，且对应的色谱保留时间和光谱信息相同；电喷雾质谱分析表明，荞麦籽粒乙醇提取物中抗氧化成分的分子量和质谱碎裂行为分别与对照品芦丁和槲皮素的相同。【结论】采用HPLC-MS/MS法可以快速筛选荞麦籽粒提取物中的抗氧化成分，荞麦籽粒提取物中的抗氧化成分主要为芦丁和槲皮素，其中槲皮素的抗氧化活性高于芦丁。
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| 关 键 词： | 荞麦 抗氧化成分 HPLC MS/MS |
| 收稿时间： | 2007-8-12 |
Studies on the Antioxidants of Ethanolic Extracts in Buckwheat by HPLC/ESI-MS |
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| YAO Ya-ping,TIAN Cheng-rui,CAO wei.Studies on the Antioxidants of Ethanolic Extracts in Buckwheat by HPLC/ESI-MS[J].Scientia Agricultura Sinica,2008,41(7):2092-2097. |
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| Authors: | YAO Ya-ping TIAN Cheng-rui CAO wei |
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| Abstract: | Objective] To study the main antioxidants in common buckwheat and tartary buckwheat .
Method] It takes the buckwheat ethanol extraction subtracts with the DPPH free radicals reaction, identify the antioxidants in the extrcats with HPLC, study the structure character of antioxidation compositions by Electro Spray Ionization-Mass Spectrometry (ESI-MS), and determine the antioxidation compositions of buckwheat ethanol extracts.
Result] Both the common buckwheat and the tartary buckwheat ethanol extracts can scavenge free radical. From the HPLC figure there were two main peaks, which included two kinds of antioxidants in the extracts. Meanwhile, the corresponding chromatograph retention time was the same as the spectrum information. The ESI-MS indicated that, the molecular weights and MS fragmentation pattern of antioxidants in the extracts were similar to the reference material of rutin and quercetin.
Conclusion] The method of HPLC-MS/MS can be used to screen the antioxidants in the extraction quickly; the main antioxidants of the buckwheat extracts were rutin and quercetin, while the activity of antioxidant activity of quercetin was higher than rutin. |
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| Keywords: | Buckwheat Antioxidant HPLC MS/MS |
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