| 贯叶连翘无菌苗茎尖诱导变异体研究 |
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| 引用本文： | 王亦菲,陆瑞菊,周润梅,孙月芳,单丽丽,黄剑华.贯叶连翘无菌苗茎尖诱导变异体研究[J].中国农学通报,2007,23(6):157-157. |
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| 作者姓名： | 王亦菲 陆瑞菊 周润梅 孙月芳 单丽丽 黄剑华 |
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| 作者单位： | 上海市农业科学院农业生物技术研究所,上海,201106;上海市农业遗传育种重点实验室,上海,201106 |
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| 基金项目： | 上海市农业科学院发展基金资助项目,贯叶连翘遗传改良新途径的研究,（2004-01-013）. |
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| 摘 要： | 以研究贯叶连翘离体诱变及筛选变异体为目的，用贯叶连翘无菌苗茎尖为供试材料，研究了平阳霉素处理浓度、时间、外源添加物对供试茎尖存活率以及激素配比对茎尖不定芽诱导率的影响。结果表明：平阳霉素80mg/L的20min处理可作为适宜诱变处理，添加椰汁有利于茎尖存活；6-BA0.5mg/L、NAA0.01mg/L的激素组合有利于诱导正常型不定芽和获得较高的诱导率。通过研究，建立了贯叶连翘变异体诱导技术程序，获得了金丝桃素含量提高的变异体材料。
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| 关 键 词： | 贯叶连翘 平阳霉素 茎尖 变异体 金丝桃素 |
| 修稿时间： | 2007-03-082007-04-06 |
Induction and selection variants using stem apexes of Hypericum perforatum L. |
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| Wang Yifei,Lu Ruiju,Zhou Runmei,Sun Yuefang,Shan Lili,Huang Jianhua.Induction and selection variants using stem apexes of Hypericum perforatum L.[J].Chinese Agricultural Science Bulletin,2007,23(6):157-157. |
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| Authors: | Wang Yifei Lu Ruiju Zhou Runmei Sun Yuefang Shan Lili Huang Jianhua |
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| Institution: | Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106; Shanghai Key Lab. of Agrieultural Genetics and Breeding, Shanghai 201106 |
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| Abstract: | The purpose of this research is to induction and selection variants of Hypericum perforatum L. Using stem apexes of Hypericum perforatum as material, effects of Pingyangmycin (PYM) concentration and treating time as well as supplementary materials in medium on survival rate of stem apexes and influence of hormone combinations on induction rate of adventitious-buds from stem apexes were investigated. The results showed that the treatment of 80mg/L PYM for 20min was suitable for mutation. The survival rate of stem apexes could be improved by adding CW in medium and the combination of hormones 0.5mg/L BA with 0.01mg/L NAA was suitable for inducing normal adventitious buds and obtaining higher induction rate. The procedure for inducing and selecting variants from stem apexes of Hypericum perforatum was established, and a variant with higher hypericin was obtained. |
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| Keywords: | Hypericum perforatum L Pingyangmycin Stem apexes Variant Hypericin |
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