Motility of spermatozoa obtained from testes of Japanese pearl oyster <Emphasis Type="Italic">Pinctada fucata martensii</Emphasis> |
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| Authors: | Hiromi Ohta Takayuki Kawamoto Kiyoshi Isowa Hideo Aoki Masahiro Hayashi Teruyoshi Narita Akira Komaru |
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| Institution: | (1) Department of Fisheries, Graduate School of Agriculture, Kinki University, 631-8505 Nara, Japan;(2) Mie Prefectural Fish Farming Center, Hamajima, 517-0404 Shima, Mie, Japan;(3) Fisheries Research Division, Mie Prefectural Science and Technology Promotion Center, Hamajima, 517-0404 Shima, Mie, Japan;(4) Graduate School, Faculty of Bioresources, Mie University, 514-8507 Tsu, Mie, Japan |
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| Abstract: | Effects of NH3 concentration in sea water and pH of sea water on the motility of spermatozoa obtained from testes were examined in the Japanese
pearl oyster Percent motility at 30 s after dilution increased with increasing NH3 concentration in sea water from 0.75–2.0 mM. When spermatozoa were diluted with sea water containing 0.75 mM NH3, which is widely used as the insemination fluid in the hatchery of this species, the percent motility increased with time
elapsed after dilution, and peaked at 5 min. For spermatozoa diluted with sea water containing 2.0 mM NH3, the percent motility increased rapidly and peaked at 30 s. The pH of sea water increased with increasing NH3 concentration from 8.2 (0 mM NH3) to 9.9 (5.0 mM NH3). When spermatozoa were diluted with artificial sea water at various pH (buffered without NH3 at 6.0–10.0), only spermatozoa diluted with artificial sea water of pH 10.0 were motile, and the percent was considerably
lower than those in ammonical sea water. These results indicate that sea water containing 2.0 mM NH3 is a suitable solution for evaluating sperm motility, and that NH3 and/or ammonium ions may activate sperm motility in this species. |
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| Keywords: | ammonical seawater artificial fertilization Japanese pearl oyster sperm motility |
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