| 灭多威降解菌mdw-1的分离和降解特性研究 |
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| 作者姓名： | XU Jing-Liang WU Jun WANG Zhi-Chun WANG Kun LI Meng-Ying JIANG Jian-Dong HE Jian LI Shun-Peng |
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| 作者单位： | XU Jing-Liang,LI Shun-Peng(Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture,Nanjing Agricultural University,Nanjin9 210095 China;Guangzhou Institute of Energy Conversion,Key Laboratory of Renewable Energy and Gas Hydrate,Chinese Academy of Sciences,Guangzhou 510640 China);WU Jun,WANG Zhi-Chun,WANG Kun,LI Meng-Ying,JIANG Jian-Dong,HE Jian(Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture,Nanjing Agricultural University,Nanjin9 210095 China)
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划)，National Program for Sci-Tech Basic Platform Construction of China，Program for Science and Technology Development of Jiangsu Province |
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| 摘 要： | Methomyl, an extremely toxic pesticide, is widely used in agriculture. A strain named mdw-1 capable of degrading methomyl rapidly was successfully isolated from activated sludge in this study. It could utilize methomyl as the sole carbon or nitrogen source. The optimal temperature and medium pH for its growth and methomyl biodegradation were 30-C and 7.0, respectively. It was identified as a Paracoccus sp. according to its morphological features, physiological and biochemical characteristics, and phylogenetic analysis based on the sequence of 16S rDNA. Gas chromatography-mass spectrometry (GC-MS) analysis showed that methomyl could be completely transformed to S-methyl-N-hydroxythioacetamidate in 10 h of incubation with the isolate mdw-1.
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| 关 键 词： | 降解 分离 鉴定 系统发育分析 生理生化特性 rDNA序列 藻 活性污泥法 |
| 收稿时间： | 21 June 2008 |
Isolation and characterization of a methomyl-degrading Paracoccus sp. mdw-1 |
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| XU Jing-Liang,WU Jun,WANG Zhi-Chun,WANG Kun,LI Meng-Ying,JIANG Jian-Dong,HE Jian,LI Shun-Peng.Isolation and characterization of a methomyl-degrading Paracoccus sp. mdw-1[J].Pedosphere,2009,19(2):238-243. |
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| Authors: | XU Jing-Liang WU Jun WANG Zhi-Chun WANG Kun LI Meng-Ying JIANG Jian-Dong HE Jian and LI Shun-Peng |
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| Institution: | [1]Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095 China [2]Guangzhou Institute of Energy Conversion, Key Laboratory of Renewable Energy and Gas Hydrate, Chinese Academy of Sciences, Guangzhou 510640 China |
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| Abstract: | Methomyl,an extremely toxic pesticide,is widely used in agriculture.A strain named mdw-1 capable of degrading methomyl rapidly was successfully isolated from activated sludge in this study.It could utilize methomyl as the sole carbon or nitrogen source.The optimal temperature and medium pH for its growth and methomyl biodegradation were 30 ℃ and 7.0,respectively.It was identified as a Paracoeeus sp.according to its morphological features,physiological and biochemical characteristics,and phylogenetic analysis based on the sequence of 16S rDNA.Gas chromatography-mass spectrometry (GC-MS) analysis showed that methomyl could be completcly transformed to S-methyl-N-hydroxythioacetamidate in 10 h of incubation with the isolate mdw-1. |
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| Keywords: | methomyl degradation Paracoceus sp S-methyl-N-hydroxythioacetamidate 16S rDNA strain mdw-1 |
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