| 大豆抗病基因同源序列的克隆与分析 |
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| 引用本文： | 丁海,宛煜嵩,朱美霞,方宣钧.大豆抗病基因同源序列的克隆与分析[J].分子植物育种,2003,1(2):217-223. |
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| 作者姓名： | 丁海 宛煜嵩 朱美霞 方宣钧 |
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| 作者单位： | 海南省热带农业资源开发利用研究所,海南省农作物分子育种重点实验室,三亚,572025 |
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| 基金项目： | 国家 8 6 3计划 (项目编号 :2 0 0 1AA2 1110 1)，国家转基因植物专项 (项目编号 :J99-A - 0 0 9)，海南省重点科技项目 (项目编号 :0 12 0 2 )资助 |
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| 摘 要： | 本研究根据已知抗病基因的NBS保守序列区设计4对简并引物和1对特异引物，以大豆农家种兴县灰布支黑豆为材料，应用PCR方法获得了11条来自基因组DNA的RGA序列和2条来自cDNA的RGA序列，序列长度在500—633bp之间，其中8条来自基因组DNA和2条来自cDNA的RGA序列已在GeneBank登录(登录号为：AF305388—305392，AY008380—008382，AY048863-AY048864)。13条序列都不同程度的含有NBS保守区的P-环(GGVGKTT)、kinase-2(VLDD)、kinase-3(GSRII)及跨膜区GLPL等特征序列结构，由此推导出的氨基酸序列同已知抗病基因L6、RPMl、SRPS2、N编码的氨基酸序列表现出从25％——42％的同源性。本研究克隆的RGA序列根据其相似性可分为4组，与已发表的大豆抗病类似基因(RLG)具有较高的相似性。
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| 关 键 词： | 大豆 抗病基因 基因克隆 序列分析 PCR 抗病基因类似物 |
Cloning and Analyzing of Resistance Gene Analogs in Soybean ZDD2315 |
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| Ding Hai Wan Yusong Zhu Meixia Fang Xuanjun Hainan Provincial Institute of Tropical Agricultural Resources,Hainan Provincial Key Laboratory of Crop Molecular Breeding,Sanya, Corresponding author,xuanjunfang@vip.sina.com.Cloning and Analyzing of Resistance Gene Analogs in Soybean ZDD2315[J].Molecular Plant Breeding,2003,1(2):217-223. |
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| Authors: | Ding Hai Wan Yusong Zhu Meixia Fang Xuanjun Hainan Provincial Institute of Tropical Agricultural Resources Hainan Provincial Key Laboratory of Crop Molecular Breeding Sanya Corresponding author xuanjunfang@vipsinacom |
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| Institution: | Ding Hai Wan Yusong Zhu Meixia Fang Xuanjun* Hainan Provincial Institute of Tropical Agricultural Resources,Hainan Provincial Key Laboratory of Crop Molecular Breeding,Sanya,572025 *Corresponding author,xuanjunfang@vip.sina.com |
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| Abstract: | Based on the NBS conserved sequences of known resistance genes, a pair of specific primer and four pairs of degenerate primers were designed to be used for amplifying soybean resistance gene analogs (RGAs). In this study, eleven RGAs from genomic DNA and two RGAs from cDNA were obtained in soybean ZDD2315 (Glycine max), which of ten sequences have been registered in the Genebank,(Accession number: AF305388-92, AY008380-82, AY048863-64). Sequence analysis of cloned RGAs indicated that the size of cloned RGAs are between 500bp to 633bp in length and four motifs of NBS conserved regions exist in the sequenced RGAs, which including P-loop (GGVGKTT), kinase-2(VLDD), kinase-3(GSRII) and membrane spanning region (GLPL). The amino acid sequences deduced from the sequenced RGAs show 25%-42% homology with amino acid sequences of L6?RPM1?SRPS2?N genes. These cloned RGAs could be divided into four gounps and have more similarity compared with the published RLGs(Resistance Like Gene). |
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| Keywords: | Soybean Glycine max Resistance gene Resistance gene analogs (RGA) |
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