| 一种东北虎全血DNA抽提的方法 |
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| 引用本文： | 黄志宏,黎绘宏,王兴金,李婉萍,宾艳芳.一种东北虎全血DNA抽提的方法[J].野生动物,2007,28(1):42-44. |
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| 作者姓名： | 黄志宏 黎绘宏 王兴金 李婉萍 宾艳芳 |
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| 作者单位： | 1. 广州动物园,广州,510070
2. 华南农业大学,广州,510642 |
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| 基金项目： | 广东省广州市科技局科技攻关项目 |
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| 摘 要： | 以8只东北虎全血为材料，用CTAB自配细胞裂解液和细胞沉淀液，采用改进的方法提取基因组DNA。结果表明，提取的基因组DNA纯度高，用琼脂糖凝胶电泳检测基因组DNA，主带清晰、无拖尾现象。
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| 关 键 词： | 东北虎 DAN抽提 全血 |
| 文章编号： | 1000-0127(2007)01-42-03 |
A Method of DNA Extraction from the Whole Blood of Siberian Tiger ( Panthera tigris altaica ) |
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| Authors: | Huang Zhihong Li Huihong Wang Xingjin Li Wanping Bin Yanfang |
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| Institution: | 1.Guangzhou Zoo, Guangzhou, 510070, China;2.South China Agricultural University, Guangzhou , 510642, China |
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| Abstract: | The genomic DNA of Siberian tiger was extracted from the whole blood by an improved method. CTAB constituted the major gradient of cell lysis and precipitation solutions, The agarose gel electrophoresis of extracted DNA showed that bands of genomic DNA were clear and no smear phenomenon was observed, indicating its high purity. |
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| Keywords: | CTAB |
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