| 香蕉枯萎病拮抗菌的筛选鉴定及其生防效果 |
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| 引用本文： | 俞鲁,凌宁,张楠,沈其荣,黄启为.香蕉枯萎病拮抗菌的筛选鉴定及其生防效果[J].南京农业大学学报,2012,35(4):81-86. |
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| 作者姓名： | 俞鲁 凌宁 张楠 沈其荣 黄启为 |
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| 作者单位： | 南京农业大学江苏省固体有机废弃物资源化高技术研究重点实验室/农业部长江中下游植物营养与肥料重点实验室,江苏南京,210095 |
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| 基金项目： | 国家公益性行业(农业)科研专项，农业部948项目重点项目(滚动项目) |
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| 摘 要： | 以海南发病香蕉园中的健康香蕉根际土壤为样品,以香蕉枯萎病病原菌Fusarium oxysporm f.sp.cubense(FOC)为指示菌进行香蕉枯萎病拮抗菌的分离筛选和鉴定,初筛得到16株拮抗菌,通过复筛得到1株有较强抑菌活性的拮抗菌株YL-19,其发酵液抑菌圈直径为26.26 mm。通过形态特征观察、生理生化鉴定及16S rDNA序列分析将其初步鉴定为芽孢杆菌属(Bacillus sp.)。该菌最适生长温度为37～42℃,最适初始pH值为6～8。对发酵液的抑菌物质稳定性初步研究显示:抑菌物质对胰蛋白酶及紫外线照射不敏感,对强酸、强碱和温度比较敏感。盆栽试验结果表明:用YL-19二次发酵的生物有机肥对香蕉枯萎病的防治效果达到74%,且对香蕉苗有明显的促生效果。
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| 关 键 词： | 香蕉枯萎病 拮抗细菌 筛选 防治 芽孢杆菌 |
Isolation, identification and biocontrol effect of antagonistic bacteria against Fusarium oxysporum f.sp.cubense |
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| YU Lu
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LING Ning
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ZHANG Nan
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SHEN Qi-rong
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HUANG Qi-wei.Isolation, identification and biocontrol effect of antagonistic bacteria against Fusarium oxysporum f.sp.cubense[J].Journal of Nanjing Agricultural University,2012,35(4):81-86. |
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| Authors: | YU Lu LING Ning ZHANG Nan SHEN Qi-rong HUANG Qi-wei |
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| Institution: | (Jiangsu Key Laboratory for Solid Organic Waste Utilization/Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River,Ministry of Agriculture,Nanjng Aricultural University,Nanjing 210095,China) |
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| Abstract: | In this experiment,sixteen antagonistic strains against Fusarium oxysporum f.sp.cubense(FOC)were screened from banana rhizosphere soils collected from the experimental plot of banana in Hainan Province.One of the isolated strains,YL-19,which showed the strongest antifungal activity,was selected for the further study.The diameter of its inhibition zone against FOC was above 26.26 mm.According to the observations of morphological characters,physiological and biochemical tests,and 16S rDNA sequence analysis,the strain YL-19 was identified to be Bacillus sp.The optimal growth temperature and pH was 37-42 ℃ and 6-8,respectively.The antibacterial substances in the fermentation broth of YL-19 were resistant to trypsin,ultraviolet radiations and base,but sensitive to acid,base and heat.Pot experiment showed that the application of bioorganic fertilizers made of YL-19 at the seedling stage of banana could decrease the disease incidence of FOC by 74%,compared with control,and had significant growth-promoting effect on banana plants. |
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| Keywords: | banana fusarium oxysporum wilt antagonistic bacterium selection control Bacillus sp |
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