| 响应曲面法制备甘草次酸脂质体工艺优化 |
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| 引用本文： | 赵晓娟,范云鹏,王梦琳,赵翰飞,李彬,胡元亮,袁菊,王德云.响应曲面法制备甘草次酸脂质体工艺优化[J].南京农业大学学报,2012,35(4):99-104. |
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| 作者姓名： | 赵晓娟 范云鹏 王梦琳 赵翰飞 李彬 胡元亮 袁菊 王德云 |
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| 作者单位： | 南京农业大学中兽医学研究室,江苏南京,210095 |
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| 基金项目： | 国家自然科学基金，中央高校基本科研业务费专项资金项目，国家SRT计划项目，南京农业大学SRT计划项目 |
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| 摘 要： | 为采用薄膜分散法制备甘草次酸脂质体,在单因素试验的基础上,采用响应曲面法研究了大豆磷脂与甘草次酸质量比(脂药比)、大豆磷脂与胆固醇质量比(膜材比)和水浴温度3个因素对脂质体包封率的影响。经过Design-Expert 7.0软件分析数据,得到脂质体的最佳制备条件:脂药比为9∶1,膜材比为2.5∶1,水浴温度为31℃。在最佳制备条件下进行验证试验,包封率实测值为(83.46±0.55)%,与包封率预测值相比,相对误差仅为0.47%。结论:Box-Behnken Design结合响应曲面分析法可以很好地对甘草次酸脂质体工艺进行优化。
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| 关 键 词： | 甘草次酸脂质体 薄膜分散法 响应曲面法 制备工艺 包封率 |
Optimization on preparation condition of glycyrrhetinic acid liposome by response surface methodology |
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| ZHAO Xiao-juan
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FAN Yun-peng
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WANG Meng-lin
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ZHAO Han-fei
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LI Bin
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HU Yuan-liang
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YUAN Ju
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WANG De-yun.Optimization on preparation condition of glycyrrhetinic acid liposome by response surface methodology[J].Journal of Nanjing Agricultural University,2012,35(4):99-104. |
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| Authors: | ZHAO Xiao-juan FAN Yun-peng WANG Meng-lin ZHAO Han-fei LI Bin HU Yuan-liang YUAN Ju WANG De-yun |
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| Institution: | (Institute of Traditional Chinese Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | Glycyrrhetinic acid(GA)liposome was prepared by thin-film dispersion method.Based on the single-factor experiment,the influence of ratio of soyben phosphatide to drug,ratio of soyben phosphatide to cholesteral and water bath temperature on the entrapment rate(ER)was studied by response surface methodology.The data was analyzed with Design-Expert 7.0 software,the optimum preparation conditions were as follows:ratio of soyben phosphatide to GA was 9∶1,ratio of soyben phosphatide to cholesteral was 2.5∶1,and water temperature was 31 ℃.Verification tests were carried out under the optimal preparation condition and the average of ER was(83.46±0.55)%.Compared with the predicted value,the relative error was only 0.47%.The Box-Behnken Design and response surface analysis can well optimize the preparation conditions of glycyrrhetinic acid liposome. |
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| Keywords: | glycyrrhetinic acid liposome thin-film dispersion method response surface methodology preparation conditions entrapment rate |
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