| 转基因抗虫油菜中Bt杀虫蛋白基因稳定遗传和高效表达及抗虫性研究 |
| |
| 引用本文： | 林良斌,官春云,周小云,何业华,杨志新.转基因抗虫油菜中Bt杀虫蛋白基因稳定遗传和高效表达及抗虫性研究[J].作物学报,2002,28(2):175-178. |
| |
| 作者姓名： | 林良斌 官春云 周小云 何业华 杨志新 |
| |
| 作者单位： | 云南农业大学烟草种植技术学院,云南昆明,650201 |
| |
| 基金项目： | 湖南省科委基金项目 (97JKY10 0 5 ) |
| |
| 摘 要： | 通过对转Bt杀虫蛋白基因油菜植株的后代进行卡那霉素抗性分析和PCR技术检测,结果表明:Bt杀虫蛋白基因是以单拷贝、 杂合地整合到转基因植株(T01、 T02、 T03、 T05、 T06、 T07、 T08)的基因组中,并稳定地遗传.ELISA检测表明:在第3～第9叶期Bt杀虫蛋白基因在转基因油菜植株中高效地表达,Bt杀虫蛋白的表达量为170～260 ng/25 m
|
| 关 键 词： | 转基因油菜 Bt杀虫蛋白基因 遗传 表达 抗虫性 |
| 收稿时间： | 2000-11-07 |
| 修稿时间： | 2000年11月7日 |
Stable Heredity and Efficient Expression of Bt Insecticidal Protein Gene in the Transgenic Rapeseed and Its Insect-resistant Activity |
| |
| LIN Liang Bin,GUAN Chun Yun,ZHOU Xiao Yun,HE Ye Hua,YANG Zhi Xin.Stable Heredity and Efficient Expression of Bt Insecticidal Protein Gene in the Transgenic Rapeseed and Its Insect-resistant Activity[J].Acta Agronomica Sinica,2002,28(2):175-178. |
| |
| Authors: | LIN Liang Bin GUAN Chun Yun ZHOU Xiao Yun HE Ye Hua YANG Zhi Xin |
| |
| Institution: | LIN Liang Bin 1 GUAN Chun Yun 2 ZHOU Xiao Yun 2 HE Ye Hua 2 YANG Zhi Xin 1 |
| |
| Abstract: | The progeny of transgenic rapeseed plants with Bt insecticidal protein gene was detected by kana mycin sifting and PCR. Results showed that single copy of Bt insecticidal protein gene has been integrated into the genome of T 0 transgenic plants and stably inherited.By ELISA Bt insecticidal protein gene can efficiently express in the transgenic rapeseed plants with the 3th~9th leaf stage, and the expressing quantity is 170~260ng/25mg fresh leaf.It amounts to 0.067%~0.105% of plant soluble protein,and the insecticidal activity is 72.0%~99.4%.But expressing quantity of Bt insecticidal protein apparently decreased after the 11th leaf stage and down to 7~50ng/25mg fresh leaf,corresponding to 0.003%~0.02% of plant soluble protein. |
| |
| Keywords: | Transgenic rapeseed Bt insecticidal protein gene Heredity Expression Insect resistant activity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《作物学报》浏览原始摘要信息 |
| 点击此处可从《作物学报》下载免费的PDF全文 |
