| Genefinder和EB在DNA电泳谱带定量分析中的比较 |
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| 引用本文： | 崔成德,王克林,李涢,袁朝阳.Genefinder和EB在DNA电泳谱带定量分析中的比较[J].安徽农业科学,2008,36(2):452-453. |
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| 作者姓名： | 崔成德 王克林 李涢 袁朝阳 |
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| 作者单位： | 中国中医科学院基础理论研究所,北京,100700;中国中医科学院基础理论研究所,北京,100700;中国中医科学院基础理论研究所,北京,100700;中国中医科学院基础理论研究所,北京,100700 |
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| 基金项目： | 国家中医药管理局归国人员科技活动择优项目 |
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| 摘 要： | 目的]比较Genefinder和EB对DNA电泳谱带定量分析的影响。方法]用加入Genefinder或EB的琼脂糖凝胶进行PBR322DNA电泳,并用凝胶成像分析系统进行标准曲线相关性等分析。结果]用Genefinder作染料,标准曲线相关性、样品含量百分误差、灵敏度等都比EB好。结论]用Genefinder作染料不仅提高了定量分析的质量,而且安全、无污染,有利于保护环境。
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| 关 键 词： | PBR322DNA Genefinder EB 凝胶成像分析系统 |
| 文章编号： | 0517-6611（2008）02-00452-02 |
| 收稿时间： | 2007-09-19 |
| 修稿时间： | 2007年9月19日 |
Comparision of Genefinder with EB in a Quantitative Analysis of a DNA Band |
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| CUI Cheng-de et al.Comparision of Genefinder with EB in a Quantitative Analysis of a DNA Band[J].Journal of Anhui Agricultural Sciences,2008,36(2):452-453. |
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| Authors: | CUI Cheng-de |
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| Abstract: | Objective] The aim was to research the effect of the DNA band dyed by Genefinder and EB on quantitative analysis in electrophoresis. Method] PBR322 DNA electrophoresis was made on an agarose gel with Genefinder or EB. Quantitative analysis was finished by gel imaging and analysis system.Result] Standard curve correlation, the percentage error of the sample and the sensitivity were better by using Genefinder instead of EB.Conclusion] It was not only improved the quality of quantitative analysis, but also protected against pollution of environment with Genefinder. |
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| Keywords: | PBR322 DNA Genefinder EB Gel imaging and analysis system |
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