| 马铃薯纺锤块茎类病毒的检测和防治 |
| |
| 引用本文： | 崔荣昌,李芝芳,李晓龙,王国学.马铃薯纺锤块茎类病毒的检测和防治[J].植物保护学报,1992,19(3):263-269. |
| |
| 作者姓名： | 崔荣昌 李芝芳 李晓龙 王国学 |
| |
| 作者单位： | 黑龙江省农业科学院马铃薯研究所 克山 |
| |
| 摘 要： | 马铃薯纺锤块茎类病毒病(potato spindle tuber viroid,PSTVd)是一种严重为害马铃薯生产的病害,降低产量20%—30%。防治的主要措施是应用无类病毒的种薯。由于目前还没有脱掉类病毒的有效措施,只能从未被饱和侵染的群体中鉴定筛选出未被侵染的个体,再脱掉其它病毒,作为核心繁殖材料。1987年以来,利用自制的电泳设备,以往复聚丙烯酰胺凝胶电泳法(return-polyacrylamide gel electrophoresis,R-PAGE)检测类病毒,筛选出未感病的个体,再用茎尖组织培养法脱掉其它病毒。经用马铃薯卷叶病毒等8种病毒酶标抗体鉴定筛选,获得既无类病毒也无主要马铃薯病毒的克新1、2、3和4号等主栽马铃薯品种的核心种。并已提供给省内外的良种场繁殖推广。1989和1990年抽样检测克山良种场繁殖的原种、一级和二级良种,未检测到类病毒。
|
| 关 键 词： | 马铃薯 病毒 电泳法 病害 防治 |
IDENTIFICATION OF POTATO SPINDLE TUBER VIROID(PSTVD) AND ITS CONTROL |
| |
| Cui Rongchang,Li Zhifang,Li Xiaolong and Wang Guoxue.IDENTIFICATION OF POTATO SPINDLE TUBER VIROID(PSTVD) AND ITS CONTROL[J].Acta Phytophylacica Sinica,1992,19(3):263-269. |
| |
| Authors: | Cui Rongchang Li Zhifang Li Xiaolong and Wang Guoxue |
| |
| Institution: | Potato Research Institute of Heilongjiang Academy of Agricultural Sciences, Keshan,Potato Research Institute of Heilongjiang Academy of Agricultural Sciences, Keshan,Potato Research Institute of Heilongjiang Academy of Agricultural Sciences, Keshan and Potato Research Institute of Heilongjiang Academy of Agricultural Sciences, Keshan |
| |
| Abstract: | Potato spindle tuber viroid(PSTVd)is widely spreaded in table-stock potato fields of Heilongjiang Province and caused yield losses of20%-30%.Principal control procedure is to use seed tuber known to befree from PSTVd,such as goverment-inspected certified seed.Selectionof nuclear propagating materials free from PSTVd can only be carriedout slowly because there is no quick procedure for viroid identification.Since 1987,viroid was tested by return-polyacrylamide gel electrophore-sis(R-PAGE)using simpler equipment and obtained results as good asusing manufactured equipment.Selections of seed materials free fromviroid and main potato viruses were carried out by R-PAGE and ELISAusing eight kind of enzyme-antibody conjugates including anti-PLRVantibody conjugate and the nuclear propagating materials of main potatovarieties Kexin No.1 and No.4 were obtained.From 1988,we havesupplied these nuclear propagating materials to seed potato farms ofHeilongjiang,Shanxi,Shaanxi,and Inner Mongolia and a large area ofeach classes of Elites were planted.In 1989 and 1990,identification ofnuclear materials,Elite 1 and Elite 2 sampled from Keshan secondcrop seed farm for PSTVd was carried out by R-PAGE and showednegative results.Selections free from PSTVd of nuclear propagatingmaterials of other main varieties Kexin No.2 and Kexin No.3 werealso obtained and propagated. |
| |
| Keywords: | potato spindle tuber viroid(PSTVd) return-polyacrylamide gel electrophoresis(R-PAGE) |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《植物保护学报》浏览原始摘要信息 |
| 点击此处可从《植物保护学报》下载免费的PDF全文 |
|
