| FTIR技术在烟草基因编辑素材病害初级筛选中的应用 |
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| 引用本文： | 陈建华,陈章玉,李雪梅,张承明,杨光宇,张涛,王小龙,孔光辉,王晋.FTIR技术在烟草基因编辑素材病害初级筛选中的应用[J].中国农学通报,2019,35(20):92-98. |
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| 作者姓名： | 陈建华 陈章玉 李雪梅 张承明 杨光宇 张涛 王小龙 孔光辉 王晋 |
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| 作者单位： | 1.云南中烟工业有限责任公司技术中心;2.云南民族大学;3.云南省烟草农业科学研究院 |
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| 基金项目： | 中国烟草总公司科技重大专项“基于基因组编辑的烟草工厂化育种核心技术体系构建及定向育种材料库创制”[110201601034(JY-08)]。 |
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| 摘 要： | 针对基因编辑素材出现的病害，以差异性分析为基础，围绕素材病害特征为研究核心，开发出了高通量的FTIR初级筛选方法。
通过对早花成苗期与正常成苗期烟叶研究，表明早花后烟叶糖类化合物明显增加，而蛋白质含量变化不明显，糖/蛋白质比值明显增加，糖类化合物可能是早花烟草植株的成花信号因子。黄化烟叶与正常烟叶的研究表明，黄化烟叶糖类化合物明显减少，而蛋白质含量基本不变，同时黄化烟叶中出现硝酸盐的富集，这可能是由于在黄化烟叶中，光合作用的降低，使得植物硝酸盐的吸收和同化都降低，但是同化作用降低更明显，因此，出现硝酸盐的富集。也有可能是由于编辑基因位点，阻断或降低了植物硝酸盐的同化作用。烟叶中硝酸盐的富集可以作为该类型黄化病初步筛选标准，1384 cm-1和829 cm-1为其烟叶黄化标志特征峰。此外，通过行业标准测试方法对上述化学成分变化进行了验证且分析结果一致。
主成分分析研究表明，可以明显的将上述病害烟叶与对照烟叶进行区分，并建立了相关病害的初步判定模型。
研究结果说明FTIR技术的分析结果具有较高的准确度，且不同样品间区别明显，可以用于基因编辑素材的早期病害诊断和病害分析等的高通量初级筛选。
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| 关 键 词： | PM2.5 PM2.5 PM10 气象条件 呼和浩特市 2015年 |
| 收稿时间： | 2018/8/9 0:00:00 |
| 修稿时间： | 2018/12/18 0:00:00 |
Application of FTIR Technology in the Primary Screening of Gene Editing Tobacco Material Diseases |
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| Abstract: | Aiming at the diseases that appear in gene editing tobacoo materials, based on the differential analysis, we developed a high-throughput FTIR primary screening method based on the characteristics of tobacco material diseases as the core of research.
Based on the premature flowering and normal seedling formation period study, showed that tobacco leaf carbohydrates increased significantly after premature flowering, while the protein content did not change significantly, ratio of sugar to protein increased significantly. The carbohydrates may be premature flowering tobacco plants flowering signal factor. Study on the chlorisis leaves and normal leaves showed that the carbohydrate in chlorisis leaf decreased significantly, and the protein content was basically unchanged. While the nitrate was enriched in chlorisis leaves, which may be due the photosynthesis decreased in chlorisis leaves, the absorption and assimilation of plant nitrate were decreased, but the assimilation decreased more significantly, therefore, the nitrate was enriched in chlorisis leaves. It is also possible to block or reduce the assimilation of nitrate in plants due to the editing of gene loci. The enrichment of nitrate in tobacco leaves can be used as a preliminary screening criterion for this type of chlorisis disease, and the characteristic peaks of the chlorisis tobacco were in 1384 cm-1 and 829 cm-1. In addition, the changes in the chemical composition are verified by other analytical methods and the results are consistent.
The principal component analysis showed that the disease tobacco could be distinctly distinguished from the control tobacco, and a preliminary determination model of the related diseases tobacco was established.
The results showed that the analysis results of FTIR technology had high accuracy, and the difference between them was obvious. It could be applied to high-throughput primary screening for early disease diagnosis and disease analysis of gene editing tobacco materials. |
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| Keywords: | FTIR Gene editing tobacco Premature flowering Chlorisis tobacco Primary screening |
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