| 黏虫CYP9A113基因的克隆及外源物质对基因表达的诱导效应 |
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| 引用本文： | 张雅男,刘月庆,JUNAID S M,王智琪,樊 东.黏虫CYP9A113基因的克隆及外源物质对基因表达的诱导效应[J].植物保护,2019,45(4):97-103. |
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| 作者姓名： | 张雅男 刘月庆 JUNAID S M 王智琪 樊 东 |
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| 作者单位： | 东北农业大学农学院, 哈尔滨 150030 |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目(12531045); 东北农业大学大学生创新实践训练计划项目(201810224271) |
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| 摘 要： | 黏虫是我国作物上最重要的害虫之一。细胞色素P450能够参与昆虫外源物质代谢。本研究采用RACE技术克隆了一条编码黏虫P450基因的cDNA序列,并通过Real-time PCR技术,检测了4种外源物质对该基因表达的诱导效应。该基因被国际P450命名委员会命名为CYP9A113,GenBank登录号为KY436739。利用2.5%高效氯氟氰菊酯乳油的LD_(50)处理黏虫3 h,LD_(10)、LD_(30)和LD_(50)处理12 h和24 h,可诱导表达CYP9A113基因;20%氯虫苯甲酰胺悬浮剂的LD_(10)处理黏虫12、24和48 h,LD_(30)和LD_(50)处理24 h,CYP9A113基因表达呈诱导效应;0.1和0.5 mg/mL香豆素处理6、12、24和48 h,CYP9A113基因表达均呈诱导效应;0.1和0.5 mg/mL吲哚-3-甲醇处理3、6、12、24和48 h,CYP9A113基因表达均呈诱导效应。
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| 关 键 词： | 黏虫 CYP9A113基因 克隆 外源物质 诱导效应 |
| 收稿时间： | 2018/8/16 0:00:00 |
| 修稿时间： | 2018/10/14 0:00:00 |
Cloning of CYP9A113 and inductive effect of exogenoussubstances on the gene expression in Mythimna separata |
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| ZHANG Yanan,LIU Yueqing,JUNAID S M,WANG Zhiqi,FAN Dong.Cloning of CYP9A113 and inductive effect of exogenoussubstances on the gene expression in Mythimna separata[J].Plant Protection,2019,45(4):97-103. |
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| Authors: | ZHANG Yanan LIU Yueqing JUNAID S M WANG Zhiqi FAN Dong |
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| Institution: | College of Agriculture, Northeast Agricultural University, Harbin 150030, China |
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| Abstract: | Mythimna separata is one of the most important pests in China. Cytochrome P450 participates in the metabolism of exogenous substances in insects. In this study, a cytochrome P450 cDNA sequence was cloned, and its induction by four exogenous substances were detected. The gene was named CYP9A113 by P450 Nomenclature Committee (GenBank accession no. KY436739). CYP9A113 could be induced by lambda-cyhalothrin 2.5% EC at LD50 for 3 h and LD10, LD30 and LD50 for 12 and 24 h. Treated with LD10 of chlorantraniliprole 20% SC for 12, 24, 48 h and with LD30 and LD50 for 24 h, CYP9A113 could be induced. Moreover, CYP9A113 could be induced by 0.1 and 0.5 mg/mL of indole-3-carbinol for 3, 6, 12, 24, 48 h and coumarin for 6, 12, 24 and 48 h. |
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| Keywords: | Mythimna separata CYP9A113 cloning exogenous substance inductive effect |
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