| 不同绵羊群体BMP4基因多态性与产羔数关系分析 |
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| 引用本文： | 任立坤,刘月琴,张英杰,王红娜,杨少华,董李学.不同绵羊群体BMP4基因多态性与产羔数关系分析[J].河北农业大学学报,2012,35(1):66-70. |
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| 作者姓名： | 任立坤 刘月琴 张英杰 王红娜 杨少华 董李学 |
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| 作者单位： | 1. 河北农业大学动物科学技术学院,河北保定,0710000
2. 唐山市畜牧水产品质量监测中心,河北唐山,063000 |
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| 基金项目： | 科技支撑计划(2008BADB2B04-9);转基因生物新品种培育重大专项(2008ZX08008-003-01) |
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| 摘 要： | 以BMP4基因为候选基因,采用PCR-SSCP方法,分析了小尾寒羊和无角陶赛特及其杂交F1代、F2代的BMP4基因的不同基因型频率,并对该位点不同基因型与产羔数之间的关系进行了分析。结果显示:小尾寒羊BB、B+、++基因型频率分别是1.0%、29.7%、69.2%;无角陶赛特BB、B+、++型频率分别为0、80.8%、19.2%;陶寒F1代BB、B+、++基因型频率分别为5.3%、39.4%、55.3%;陶寒F2代BB、B+、++型基因型频率分别为0、5.0%、95.0%。小尾寒羊的基因型++、B+、BB平均产羔数分别为(1.00±0.00)、(1.67±0.11)、(2.40±0.13),经χ2检验,差异极显著(P<0.01);陶寒F1代的基因型++、B+、BB平均产羔数分别为(2.00±0.00)、(2.45±0.53)、(2.00±0.00),经χ2检验,++、BB组与B+组的平均产羔数间的差异极显著(P<0.01);B基因不能提高无角陶塞特母羊产羔数。
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| 关 键 词： | BMP4基因 小尾寒羊 无角陶赛特 产羔数 |
The analysis on BMP4 gene polymorphism and litter size in different population of sheep |
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| REN Li-kun
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LIU Yue-qin
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ZHANG Ying-jie
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WANG Hong-na
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YANG Shao-hua
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DONG Li-xue.The analysis on BMP4 gene polymorphism and litter size in different population of sheep[J].Journal of Agricultural University of Hebei,2012,35(1):66-70. |
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| Authors: | REN Li-kun LIU Yue-qin ZHANG Ying-jie WANG Hong-na YANG Shao-hua DONG Li-xue |
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| Institution: | 1.College of Animal Science and Technology,Agricultural University of Hebei,Baoding 071000,China; 2.The Livestock and Aquatic Products Quality Inspection and Test Center,Tangshan 063000,China) |
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| Abstract: | Bone morphogenetic protein 4(BMP4)gene was detected in both Small Tailed Han sheep,Dorset sheep,F1 generation sheep(Small Tailed Han sheep×Dorset sheep) and F2 generation sheep(F1 generation sheep×Dorset sheep)by PCR-SSCP as a candidate gene for the prolificacy of sheep,analyzed the relationship between the sites of different genotypes and litter size.The results indicated that the genotype frequencies of BB,B+ and ++ of Small Tailed Han sheep were 1.0%,29.7% and 69.2% respectively;the genotype frequencies of B+ and ++ of Dorset sheep were 80.8% and 19.2% respectively;the genotype frequencies of BB,B+ and ++ of F1 generation sheep were 5.3%,39.4% and 55.3% respectively;the genotype frequencies of BB,B+ and ++ of F2 generation sheep were 0,5.0% and 95.0%.The average litter size of the genotype of ++,B+ and BB were(1.00±0.00),(1.67±0.11) and(2.40±0.13),and the difference was extremely significant tested by χ2 analysis(P<0.01).the genotype of ++,B+ and BB of F1 generation were(2.00±0.00),(2.45±0.53) and(2.00±0.00),and the difference of ++,BB and B+ was extremely significant(P<0.01);gene B can’t increase the litter size of Dorset sheep. |
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| Keywords: | BMP4 gene small tailed han sheep poll dorset litter size |
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