| 芦笋花药培养愈伤诱导条件优化与植株再生 |
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| 引用本文： | 陈海媛,胡淑明,乜兰春,张学英,李俊萍.芦笋花药培养愈伤诱导条件优化与植株再生[J].河北农业大学学报,2012,35(1):41-45. |
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| 作者姓名： | 陈海媛 胡淑明 乜兰春 张学英 李俊萍 |
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| 作者单位： | 河北农业大学园艺学院,河北保定,071000 |
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| 基金项目： | 河北农业大学科学发展计划(2007020) |
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| 摘 要： | 以栽培表现较好杂交一代品种‘Apollo’为试材,研究了芦笋花药培养技术,建立了一套较完善的芦笋花药培养技术体系。结果表明:1～2mm的花蕾、4℃低温预处理5d、MS+2.0mg/L 6-BA+1.0mg/L NAA为愈伤组织和芽诱导的最佳条件;最适继代增殖条件为:MS+0.5mg/L 6-BA+0.2mg/L NAA。并对继代增殖组培苗进行了初步的倍性鉴定。
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| 关 键 词： | 芦笋 花药培养 技术体系 |
Plant regeneration of Asparagus of ficinalis L.anther culture with optimized conditions for callus induction |
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| CHEN Hai-yuan
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HU Shu-ming
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NIE Lan-chun
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ZHANG Xue-ying
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LI Jun-ping.Plant regeneration of Asparagus of ficinalis L.anther culture with optimized conditions for callus induction[J].Journal of Agricultural University of Hebei,2012,35(1):41-45. |
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| Authors: | CHEN Hai-yuan HU Shu-ming NIE Lan-chun ZHANG Xue-ying LI Jun-ping |
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| Institution: | (College of Horticulture,Agricultural University of Hebei,Baoding 071000,China) |
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| Abstract: | In order to establish a better system of Asparagus anther culture technology,a plant of the first filial generation ’Apollo’ which had a better character in the process of growth was selected.The results showed that the best callus and shoots inducted conditions were buds whose lengths were 1-2 mm,storage at 4 ℃ for 5 days and a MS culture medium containing 2.0 mg/L 6-BA,1.0 mg/L NAA;the best subculture medium was MS+0.5 mg/L 6-BA+0.2 mg/L NAA.Plant regenerations from callus were ploidy identified. |
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| Keywords: | Asparagus anther culture technology system |
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