| 转基因水稻对稻瘟病的抗性研究 |
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| 引用本文： | 杨祁云,许新萍,朱小源,冯道荣,李宝健.转基因水稻对稻瘟病的抗性研究[J].植物病理学报,2003,33(2):162-166. |
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| 作者姓名： | 杨祁云 许新萍 朱小源 冯道荣 李宝健 |
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| 作者单位： | 1 广东省农业科学院植物保护研究所, 广州 510640;2 中山大学生物工程研究中心, 广州 510275 |
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| 基金项目： | 国家“863”高新技术发展计划资助项目(10101-02-02)，广东省自然科学基金(970674) |
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| 摘 要： | 采用苗期初筛、复筛、抗谱测定和田间自然诱发试验等不同鉴定方法,对经分子检测证明已整合有碱性几丁质酶基因和β-1,3-葡聚精酶基因的22个转化系的转基因水稻植株进行稻瘟病抗性鉴定研究,筛选出对稻瘟病的抗性比原种对照七丝软占有明显提高的一系列转基因水稻品系,其中表现高抗的有来自F4-9转化株系的7个品系。高抗材料的R7代品系,经室内抗谱测定及田间病圃试验结果,仍然表现高抗稻瘟病。本研究通过转基因技术,成功地将优质感病品种改良成高抗品系,研究结果证明了利用基因工程手段培育抗病水稻新品种是一个非常有希望的育种途径。
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| 关 键 词： | 转基因水稻 碱性几丁质酶基因 β-1 3-葡聚糖酶基因 稻瘟病抗性 |
| 文章编号： | 0412-0914(2003)02-0162-05 |
| 修稿时间： | 2002年6月25日 |
Study on the blast resistance of transgenic rice plants with two antifungal protein genes |
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| YANG Qi-yun,XU Xin-ping,ZHU Xiao-yuan,FENG Dao-rong,LI Bao-jian Plant Protection Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou ,China Biotechnology Research Center,Zhongshan University,Guangzhou ,China.Study on the blast resistance of transgenic rice plants with two antifungal protein genes[J].Acta Phytopathologica Sinica,2003,33(2):162-166. |
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| Authors: | YANG Qi-yun XU Xin-ping ZHU Xiao-yuan FENG Dao-rong LI Bao-jian Plant Protection Research Institute Guangdong Academy of Agricultural Sciences Guangzhou China Biotechnology Research Center Zhongshan University Guangzhou China |
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| Institution: | 1 Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;2 Biotechnology Research Center, Zhongshan University, Guangzhou 510275, China |
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| Abstract: | Twenty two transgenic rice lines with basic chitinase gene and β-1, 3-glucanase gen were screened for blast re-sistance by. conducting seedling screening, resistant spectrum test, and field resistance test in the blast are nursery. Aseries of transgenic rice plants with improved resistance were obtained. Among them, seven transgenic lines with high resis-tance were selected from transgenic rice line F4-9, and these highly resistant lines also expressed stable resistance in the R7generations based on the tests of resistant spectrum to blast fungus in greenhouse and the neck resistance in blast nursery.The transgenic rice lines with high resistance and quality were obtained successfully in this experiment. The data showedthat genetic engineering was a powerful approach to improve the resistance of rice variety to blast and alleviate the negativecorrelation between blast resistance and quality in southern rice area. |
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| Keywords: | transgenic rice plants basic chitinase gene β-1 3-glucanase gene resistance to rice blast |
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