| 家蚕cbp基因的表达与幼虫体内类胡萝卜素的水平相关 |
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| 引用本文： | 牛艳山,司马杨虎,徐世清.家蚕cbp基因的表达与幼虫体内类胡萝卜素的水平相关[J].安徽农业科学,2009,37(36):17986-17989. |
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| 作者姓名： | 牛艳山 司马杨虎 徐世清 |
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| 作者单位： | 苏州大学医学部应用生物学系,现代丝绸国家工程实验室,江苏苏州,215123;苏州大学医学部应用生物学系,现代丝绸国家工程实验室,江苏苏州,215123;苏州大学医学部应用生物学系,现代丝绸国家工程实验室,江苏苏州,215123 |
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| 基金项目： | 国家科技支撑计划重点项目 |
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| 摘 要： | 目的]调查家蚕幼虫期类胡萝卜素水平的变化及cbp基因的mRNA表达特征。方法]选取黄茧和绿茧品种家蚕3、4和5龄期幼虫的丝腺,参照Takara Trizol plus试剂说明提取总RNA,根据文献提供的cbp基因序列设计特异引物,采用RT-PCR方法调查不同茧色品种家蚕幼虫不同龄期丝腺中cbp基因mRNA的表达特征和主要组织中类胡萝卜素的水平变化。结果]cbp基因的mRNA表达,在3和5龄期各品种家蚕均维持一定水平,但在4龄期表达量很低或不表达。同一组织的紫外可见光谱扫描结果显示,4龄期家蚕丝腺类胡萝卜素的含量极低,不同茧色品种cbp基因的mRNA表达也存在差异,绿茧品种仅表达1个缺失第2外显子的转录本,而所有黄色茧品种还可表达1个序列完整的转录本。结论]家蚕体内类胡萝卜素的水平与cbp基因mRNA表达相关。
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| 关 键 词： | 天然彩色茧 类胡萝卜素结合蛋白 类胡萝卜素 |
Correlation of cbp Gene Expression in Silkworm with Carotenoid Level in Larvae |
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| NIU Yan-shan et al.Correlation of cbp Gene Expression in Silkworm with Carotenoid Level in Larvae[J].Journal of Anhui Agricultural Sciences,2009,37(36):17986-17989. |
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| Authors: | NIU Yan-shan |
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| Institution: | NIU Yan-shan et al(National Engineering Lab of Modern Silk,Department of Applied Biology,Medical College,Suzhou University,Suzhou,Jiangsu 215123) |
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| Abstract: | Objective]The study aimed to investigate the carotenoid level change and cbp gene expression character in silkworm larvae. Method]The silk glands of 3th,4th,5th instar larvae of silkworm with yellow and green cocoon strains were selected to extract their total DNA by referring to the reagent introduction of Takara Trizol plus and the special primer was designed on base of cbp gene sequence provided by the literature.The mRNA expression character of cbp gene and the change of carotenoid level in the main t... |
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| Keywords: | Natural colored cocoon CBP Carotenoid |
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