| 鸡和兔血糖调节生理差异比较 |
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| 引用本文： | 罗舒文,胡 璇,郑 铭,徐雪雪,刘雪明,仲向前,张 钰,陈国宏,徐 琪.鸡和兔血糖调节生理差异比较[J].西北农业学报,2023(12):1869-1878. |
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| 作者姓名： | 罗舒文 胡 璇 郑 铭 徐雪雪 刘雪明 仲向前 张 钰 陈国宏 徐 琪 |
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| 作者单位： | （1.扬州大学 动物科学与技术学院，江苏扬州 225009；2. 江苏省宿迁市畜牧兽医站，江苏宿迁 223800）为阐明禽类在高血糖情况下未表现出任何临床症状的内在原因，以鸡、家兔为研究对象，利用ELISA、高效液相色谱、液相色谱质谱检测等技术对两者葡萄糖调节生理差异进行比较。结果显示，ELISA检测鸡的糖化白蛋白显著高于兔，而糖化血红蛋白无显著性差异；鸡血清果糖胺含量与兔无显著性差异; 鸡血清终末糖基化产物含量极显著低于兔；高效液相色谱检测结果显示，鸡的牛磺酸含量和游离氨基酸含量显著高于兔；液相色谱质谱检测结果显示，鸡的甲基乙二醛含量与家兔无显著性差异。以上研究结果表明，鸡无慢性高血糖所表现出的有害效应的主要原因是牛磺酸和部分游离氨基酸作为羰基清除剂降低果糖胺及甲基乙二醛的含量，从而导致糖基化终末产物的降低。 |
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| 基金项目： | 江苏现代农业（肉鸡）产业技术体系（JATS［2022］163）。 |
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| 摘 要： | 旨在了解大口黑鲈(Micropterus salmoides )的种质特性，为其种质鉴定提供理论依据。采用传统形态学方法观测样品形态特征，借助肾细胞滴片空气干燥法制备染色体标本并进行核型分析，通过聚丙烯酰胺凝胶电泳法研究大口黑鲈眼晶状体乳酸脱氢酶(LDH)。结果表明：大口黑鲈体呈纺锤形，口裂大，斜裂；背部黑绿色，体侧青绿；吻端至尾鳍基部有排列成带状的黑斑。鳃盖上有3条呈放射状的黑斑；背鳍鳍式 D.VIII~XI-11~14；臀鳍鳍式A.III-9~12；左侧第一鳃弓外侧鳃耙数为6~7，鳔1室，脊椎骨总数30~32。染色体数目2n=46，核型公式为2m+2st+42t，臂数NF=48，未发现带有随体、次缢痕的染色体和异型性染色体，大口黑鲈进化上符合高位类群核型特征；眼晶状体中的LDH酶带5条，观察到LDHA、LDHB和LDHC 3个基因位点。综上所述，在对大口黑鲈进行种质鉴定时，建议采用多种种质鉴定技术手段系统化地考察和评价其种质状况。
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| 关 键 词： | 鸡 兔 血糖调节 糖基化终末产物 糖化蛋白 牛磺酸 甲基乙二醛 |
Comparison of Physiological Differences in Blood Glucose Regulation between Chickens and Rabbits |
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| LUO Shuwen,HU Xuan,ZHENG Ming,XU Xuexue,LIU Xueming,ZHONG Xiangqian,ZHANG Yu,CHEN Guohong and XU Qi.Comparison of Physiological Differences in Blood Glucose Regulation between Chickens and Rabbits[J].Acta Agriculturae Boreali-occidentalis Sinica,2023(12):1869-1878. |
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| Authors: | LUO Shuwen HU Xuan ZHENG Ming XU Xuexue LIU Xueming ZHONG Xiangqian ZHANG Yu CHEN Guohong and XU Qi |
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| Abstract: | The fasting blood glucose concentrations of birds are about twice of themammals, and the birds have significant insulin resistance, but there is no significant diabetic complications, the reasons for the absense are unclear.In order to elucidate the intrinsic reasons for no clinical signs of hyperglycaemia inthe poultry, this study was conducted to compare the physiological differences in glucose regulation between chickens and rabbits using ELISA, high performance liquid chromatography and liquid chromatography mass spectrometry detection techniques.The results showed that glycosylated albumin was higher in chickens than in rabbits, while glycosylated haemoglobin was not significantly different; serum fructosamine levels inchickens were not significantly different from those in rabbits; and serum levels of advanced glycation end-products were significantly lower in chickens than in rabbits .The results of high performance liquid chromatography showed that the taurine content and free amino acid content of chickens were extremely significantly higher than those of rabbits; the results of liquid chromatography mass spectrometry showed that the methylglyoxal content of chickens was not significantly different from that of rabbits.In conclusion, the main reason forchickens to exhibite deleterious effects under the no chronic hyperglycaemiais that taurine and some free amino acids act as carbonyl scavengers to reduce the levels of fructosamine and methylglyoxal, and decrease the advanced glycation end-products. |
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| Keywords: | Chicken Rabbit Glucose regulation Glycosylation end products Glycated proteins Taurine Methylglyoxal |
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