| 耐乙草胺促生菌筛选及其对玉米幼苗的促生效应 |
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| 作者姓名： | 张凤麟 黄宁 李雷 陈伟东 季欣悦 包英哲 王鸿斌 |
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| 作者单位： | 吉林农业大学资源与环境学院, 长春 130118;农业农村部盐碱土改良与利用(东北内陆盐碱地)重点实验室, 长春 130118;吉林省商品粮基地土壤资源可持续利用重点实验室, 长春 130118;吉林省公主岭市植检植保站, 吉林 公主岭 136100 |
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| 基金项目： | 吉林省科技发展计划项目（20210202023NC，20220202029NC） |
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| 摘 要： | 分离筛选具有耐乙草胺特性的促生菌株，并对其进行菌种鉴定，可为推进多功能微生物菌剂的研发提供可行性方案和理论依据。本研究利用不同浓度乙草胺的改良培养基筛选出具有高耐乙草胺能力的菌株，测试其在不同培养基条件下的促生作用，并通过促发芽试验和盆栽试验验证可行性。结果表明：菌株JL7与JL16为高耐乙草胺且具有较强促生能力的根际促生菌，两株菌对乙草胺的最大抗性浓度达700 mg·L-1。JL7的溶磷能力高达223.21 mg·L-1，产IAA（吲哚乙酸）量达82.40 mg·L-1。经鉴定JL7菌株为肠杆菌属（Enterbacter sp.）,JL16菌株为醋菌属（Acetobacter sp.）。促发芽试验结果表明，施入JL7与JL16菌株72 h后发芽率分别较对照提高9.42、3.84个百分点，且JL16菌株对芽长与根长有显著的促生作用，较对照增加52.53%、23.22%。盆栽试验中，JL7与JL16的添加对株高、茎粗、根直径、根长等植株形态指标均表现为促进作用，并证实菌株JL7、JL16在土壤中仍对乙草胺具有耐受性。两种耐乙草胺菌株在促生方面具有显著效果，这为后期微生物改良土壤和开发新型...
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| 关 键 词： | 乙草胺抗性 促生作用 根际促生菌 筛选 玉米幼苗 |
| 收稿时间： | 2023/2/22 0:00:00 |
Screening of acetochlor-resistance-promoting bacteria and their growth-promoting effects on maize seedlings |
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| Authors: | ZHANG Fenglin HUANG Ning LI Lei CHEN Weidong JI Xinyue BAO Yingzhe WANG Hongbin |
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| Institution: | College of Resources and Environment, Jilin Agricultural University, Changchun 130118, China;Key Laboratory of Saline-Alkali Soil Reclamation and Utilization in Northeast China, Ministry of Agriculture and Rural Affairs, Changchun 130118, China;Key Laboratory of Sustainable Utilization of Soil Resources in the Commodity Grain Bases, Changchun 130118, China;Gongzhuling Station of Quarantine and Plant Protection, Gongzhuling 136100, China |
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| Abstract: | Acetochlor-tolerant growth-promoting bacteria were isolated and identified to develop a microbial agent and provide a feasible scheme along with a theoretical basis for this development. Highly acetochlor-tolerant bacteria were isolated from modified media with different acetochlor concentrations. The growth-promoting effect on maize was tested, and the feasibility was verified through germinationpromoting and potting experiments. Strains JL7 and JL16 showed a strong ability to induce high acetochlor tolerance and promote growth; moreover, the maximum acetochlor resistance for both strains was achieved at a concentration of 700 mg·L-1. The phosphorus solubilization capacity of JL7 was 223.21 mg·L-1, and the cumulative production of IAA was 82.40 mg·L-1. Strains JL7 and JL16 were identified as Enterbacter sp. and Acetobacter sp., respectively. The results of the germination promotion test showed that the germination rate increased by 9.42 and 3.84 percent points with the addition of strains JL7 and JL16 after 72 hours, respectively, compared with that of the CK. Furthermore, strain JL16 significantly promoted growth in terms of shoot and root lengths by 52.53% and 23.22%, respectively, compared with those of the CK. In the pot experiment, the addition of both JL7 and JL16 affected morphological indicators such as plant height, stem thickness, root diameter, and root length, all of which were promoted. Strains JL7 and JL16 were still tolerant to ethephon in the soil. Two acetochlor-tolerant strains showed a remarkable effect on promoting plant growth. The study provides a theoretical basis for microbial soil improvements by describing suitable strains and the development of innovative microbial fertilizers for future applications. |
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| Keywords: | acetochlor resistance growth-promoting effect plant growth promoting rhizobacteria screen maize seedling |
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