| 云南怒江番茄种植新区番茄病毒检测及病毒种传特性 |
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| 引用本文： | 刘玉莹,熊志琦,苏晓霞,王田田,吴 阔,郑宽瑜,张仲凯.云南怒江番茄种植新区番茄病毒检测及病毒种传特性[J].植物保护,2024,50(1):272-279. |
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| 作者姓名： | 刘玉莹 熊志琦 苏晓霞 王田田 吴 阔 郑宽瑜 张仲凯 |
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| 作者单位： | 1. 云南省农业科学院生物技术与种质资源研究所, 云南省农业生物技术重点实验室, 昆明 650205; 2. 云南大学资源植物研究院, 昆明 650504 |
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| 基金项目： | 云南省科技计划云南种子种业联合实验室专项（202205AR070001）；云南省科技计划重大科技专项（202202AE090022）；国家自然科学基金地区科学基金（32160620） |
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| 摘 要： | 病毒病对番茄生产造成严重危害, 近年来在番茄种植新区发生严重, 疑似为种子带毒传播。本研究通过对云南省怒江州番茄种植新区的番茄病毒病样品采用RNA-seq高通量测序, RT-PCR验证的方法检测病毒种类;对番茄病果种子进行超薄切片制样透射电子显微镜观察, 将病果种子播种后对种苗进行RT-PCR带毒检测。结果表明, RNA-seq高通量测序及RT-PCR检测到的病毒有番茄环纹斑点病毒(tomato zonate spot orthotospovirus, TZSV)、番茄黄斑驳相关病毒(tomato yellow mottle-associated virus, TYMaV)、辣椒脉斑驳病毒(chili veinal mottle virus, ChiVMV)、南方番茄病毒(southern tomato virus, STV)。透射电镜观察到种胚细胞及胚乳细胞中分布典型的正番茄斑萎病毒属Orthotospovirus病毒粒体。病果种子播种28 d后的种苗具有病毒病症状, 通过RT-PCR检出TZSV、ChiVMV、STV, 检出率分别为60%、100%、80%。上述研究结果为TZSV通过种子传播提供了有利的证据, 并为源头防控番茄病毒病提供了依据。
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| 关 键 词： | 番茄 番茄病毒 病毒检测 RNA-seq 种传病毒 |
| 收稿时间： | 2022/11/29 0:00:00 |
| 修稿时间： | 2023/1/16 0:00:00 |
Detection and seed transmission characteristics of tomato viruses in tomato planting new area of Nujiang, Yunnan |
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| LIU Yuying,XIONG Zhiqi,SU Xiaoxi,WANG Tiantian,WU Kuo,ZHENG Kuanyu,ZHANG Zhongkai.Detection and seed transmission characteristics of tomato viruses in tomato planting new area of Nujiang, Yunnan[J].Plant Protection,2024,50(1):272-279. |
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| Authors: | LIU Yuying XIONG Zhiqi SU Xiaoxi WANG Tiantian WU Kuo ZHENG Kuanyu ZHANG Zhongkai |
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| Institution: | 1. Biotechnology and Genetic Germplasm Resources Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming 650205, China; 2. School of Agriculture, Yunnan University, Kunming 650504, China |
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| Abstract: | Virus diseases have caused serious harm to tomato production. In recent years, it has occurred seriously in some new tomato planting area, and the source of the viruses were suspected from the tomato seeds. In this study, RNA-seq high-throughput sequencing and RT-PCR were used to detect virus species in diseased tomato samples in the new tomato planting area of Nujiang prefecture, Yunnan province. Furthermore, the diseased tomato fruit seeds were collected for ultrathin section preparation and electron microscopy observation. After seeding of diseased tomato fruit seeds, the seedlings were detected for viruses by RT-PCR. The results showed that the viruses detected by RNA-seq and RT-PCR include tomato zonate spot orthotospovirus (TZSV), tomato yellow mottle-associated virus (TYMaV), chili veinal mottle virus (ChiVMV) and southern tomato virus (STV). The Orthotospovirus virions were observed in the embryo cells of the diseased tomato fruit seeds by transmission electron microscopy (TEM). 28 days after the diseased fruit seeds were sown, the viral like symptoms were observed on seedlings. TZSV, ChiVMV and STV were detected on seedling by RT-PCR, and the infection rates were 60%, 100% and 80%, respectively. These results provide strong evidence for TZSV transmission by seeds and provide a basis for source prevention and control of tomato virus disease. |
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| Keywords: | tomato tomato virus virus detection RNA-seq seed-transmitted virus |
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