Extraction and Characterization of Lipids from the Kernels,Leaves, and Stalks of Nine Grain Sorghum Parent Lines |
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| Authors: | K L Christiansen C L Weller V L Schlegel S L Cuppett T P Carr |
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| Institution: | 1. Abengoa Bioenergy Corporation, Engineering, St. Louis, MO.;2. University of Nebraska-Lincoln, Biological Systems Engineering.;3. University of Nebraska-Lincoln, Food Science and Technology.;4. University of Nebraska-Lincoln, Nutrition and Health Sciences. |
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| Abstract: | The objectives of this study were to identify and quantify lipid classes extracted from the stalk, leaves, and the whole kernels of nine different sorghum parent lines. All extractions were performed with a Soxtec using n-hexane as the extraction solvent. Analytical methods including high-performance liquid chromatography (HPLC), gas chromatography (GC), and thin-layer chromatography (TLC) were used to identify and quantify the extracted lipid classes: triacylglycerides (TAG), diglycerides (DG), policosanols (PA), free sterols (FS), phytosterols (PS), free fatty acids (FFA), tocopherols (T), and fatty aldehydes. Overall, extracts from the leaves had higher amounts of soluble matter. Extracts from leaves contained more alcohols and sterols, whereas extracts from the stalks and the whole kernels contained the greatest amount of free fatty acids and fatty aldehydes, respectively. Trace amounts of tocopherol were detected by HPLC in all 27 samples. The lipid profiles of extracts from the whole kernels, leaves, and stalks from the same plant differed. The lipid profiles of extracts from each anatomical part of the sorghum plant differed between the nine parent lines. |
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