| 长期碳酸盐碱度胁迫对脊尾白虾生长及卵巢发育的影响 |
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| 引用本文： | 张秀红,李吉涛,王佳佳,王成伟,秦桢,葛倩倩,刘萍,李健.长期碳酸盐碱度胁迫对脊尾白虾生长及卵巢发育的影响[J].水产学报,2024,48(4). |
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| 作者姓名： | 张秀红 李吉涛 王佳佳 王成伟 秦桢 葛倩倩 刘萍 李健 |
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| 作者单位： | 上海海洋大学 水产科学国家级实验教学示范中心,中国水产科学研究院黄海水产研究所,中国水产科学研究院黄海水产研究所,中国水产科学研究院黄海水产研究所,中国水产科学研究院黄海水产研究所,中国水产科学研究院黄海水产研究所,中国水产科学研究院黄海水产研究所,中国水产科学研究院黄海水产研究所 |
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| 基金项目： | 国家重点研发计划课题（2018YFD0901302）； 国家自然科学基金项目（32072974）； 现代农业产业技术体系（CARS-48）； 中国水产科学研究院基本科研业务费项目(2020TD46) |
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| 摘 要： | 为探讨碳酸盐碱度对脊尾白虾（Exopalaemon carinicauda）生长及卵巢发育的影响。本研究设置3 mmol/L（对照组）、5 mmol/L和8 mmol/L 3种碳酸盐碱度梯度养殖脊尾白虾60天，比较不同碳酸盐碱度胁迫对脊尾白虾生长性能、组织结构、酶活性以及卵巢发育的影响，结果显示：5 mmol/L组与对照组体长增长率、体重增长率以及成活率差异不显著（P>0.05），8 mmol/L组体长增长率、体重增长率以及成活率显著低于对照组（P<0.05）。肝胰腺组织切片显示，5 mmol/L组中部分B细胞体积增大，部分转运泡内出现颗粒物质，8 mmol/L组管腔的形态结构严重变形。鳃组织切片显示，5 mmol/L组出现轻微鳃丝肿大现象，角质层略有变形，且角质层下间隙扩张变长，上皮细胞与支柱细胞排列紊乱；8 mmol/L组鳃丝肿大，排列不规则，出现血细胞肿胀现象，鳃丝上皮细胞严重破坏，毛细血管网结构形态改变，角质层下间隙变形。肌肉组织酶活性测定结果显示，5mmol/L组的碳酸酐酶活性显著高于对照组（P<0.05），Na+/K+-ATP酶活性在肌肉中差异不显著（P>0.05）；肝胰腺组织酶活性测定结果显示，碳酸酐酶在肝胰腺组织中差异不显著（P>0.05），8 mmol/L组Na+/K+-ATP酶活性显著低于其他组（P<0.05），鳃组织中，5 mmol/L和8 mmol/L组鳃组织中和Na+/K+-ATP酶活性均显著高于对照组（P<0.05），且两组间差异显著。卵巢发育统计结果显示，三组脊尾白虾卵巢均能发育，其发育至Ⅱ期的百分比分别为20.51%、10.52%和6.25%，其中5 mmol/L组与对照组间无显著差异（P>0.05），8 mmol/L组显著低于对照组（P<0.05）；3 mmol/L组和5 mmol/L组均有卵巢发育至Ⅲ期的脊尾白虾，但两组差异不显著（P>0.05），8 mmol/L组未见有卵巢发育至Ⅲ期的脊尾白虾。卵巢组织切片显示，8 mmol/L组卵原细胞细胞排列疏松，周围滤泡细胞排列疏松且数量较少。综上所述，脊尾白虾可以在低于8 mmol/L的碳酸盐碱度中生长发育，但高碳酸盐碱度可能会造成脊尾白虾鳃和肝胰腺组织损伤，导致其生长受到影响，同时猜测高碳酸盐碱度可能也会影响卵巢的发育速度。
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| 关 键 词： | 脊尾白虾 碳酸盐碱度胁迫 生长 组织切片 酶活 卵巢发育 |
| 收稿时间： | 2022/4/1 0:00:00 |
| 修稿时间： | 2022/7/27 0:00:00 |
Effects of long-term saline-alkaline stress on growth and ovary development of Exopalaemon carinicauda |
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| Zhang Xiuhong,Li Jitao,Wang Jiaji,Wang Chengwei,Qin Zhen,Ge Qianqian,Liu Ping and Li Jian.Effects of long-term saline-alkaline stress on growth and ovary development of Exopalaemon carinicauda[J].Journal of Fisheries of China,2024,48(4). |
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| Authors: | Zhang Xiuhong Li Jitao Wang Jiaji Wang Chengwei Qin Zhen Ge Qianqian Liu Ping and Li Jian |
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| Institution: | Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences |
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| Abstract: | In order to investigate the effects of carbonate alkalinity on the growth and ovary development of the ridgetail white prawn Exopalaemon carinicauda. Three carbonate alkalinity gradients of 3 mmol/L (control group), 5 mmol/L and 8 mmol/L were set to culture E. carinicauda for 60 days. The effects of different carbonate alkalinity stress on the growth performance, tissue structure, enzyme activity and ovary development of E. carinicauda were compared. The results showed that there was no significant difference between the 5mmol/l group and the control group in growth gain, weight gain and survival rate (p>0.05), and the growth gain, weight gain and survival rate of the 8 mmol/l group were significantly lower than those of the control group (p<0.05); The tissue sections of hepatopancreas showed that the volume of some B cells increased in 5mmol/l group, and particulate matter appeared in some transport vesicles, and the morphological structure of the lumen was severely deformed in 8mmol/l group. The gill tissue section showed that in the 5 mmol/l group, there was slight swelling of gill filaments, slight deformation of the stratum corneum, expansion and lengthening of the space under the stratum corneum, and disordered arrangement of epithelial cells and pillar cells; In 8 mmol/l group, gill filaments were swollen, arranged irregularly, blood cells swelled, gill filaments epithelial cells were seriously damaged, the structure and morphology of capillary network were changed, and the space under the stratum corneum was deformed. The results of muscle tissue enzyme activity showed that the carbonic anhydrase activity of 5mmol/l group was significantly higher than that of the control group (p<0.05), and the Na+/K+-ATPase activity had no significant difference in muscle (p>0.05); The results of enzyme activity determination in hepatopancreas showed that there was no significant difference in carbonic anhydrase in hepatopancreas (p>0.05), Na+/K+-ATPase activity in 8 mmol/l group was lower than that in other groups (p<0.05), and Na+/K+-ATPase activity in gill tissue in 5 mmol/l and 8 mmol/l groups was significantly higher than that in control group (p<0.05), and the difference between the two groups was significant.; The ovaries of the three groups were able to develop, and the percentages of their development to phase II were 20.51%, 10.52% and 6.25% respectively. There was no significant difference between the 5 mmol/l group and the control group (p>0.05), and there was significant difference between the 8 mmol/l group and the control group (p<0.05); Both 3 mmol/l group and 5 mmol/l group had E. carinicauda with ovary development to stage III, but there was no significant difference between the two groups (p>0.05), and no E. carinicauda with ovary development to stage III was found in 8 mmol/l group.; Ovary tissue sections showed that the arrangement of oocytes in 8 mmol/l group was loose, and the arrangement of surrounding follicular cells was loose and the number was small. To sum up, E. carinicauda can grow and develop in a carbonate alkalinity of less than 8 mmol / L, but high carbonate alkalinity may cause damage to the gills and hepatopancreas of E. carinicauda, affecting its growth. At the same time, it is speculated that high carbonate alkalinity may also affect the development speed of ovaries. |
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| Keywords: | Exopalaemon carinicauda Carbonate alkalinity stress Growth tissue Sections Enzyme activity Ovary development |
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