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| 利用分子标记辅助选择聚合水稻基因Xa21和Pi9(t) | |
| 引用本文： | 倪大虎,易成新,李莉,汪秀峰,王文相,杨剑波.利用分子标记辅助选择聚合水稻基因Xa21和Pi9(t)[J].分子植物育种,2005,3(3):329-334. |
| 作者姓名： | 倪大虎 易成新 李莉 汪秀峰 王文相 杨剑波 |
| 作者单位： | 1. 安徽农业大学农业学院,合肥,230036;安徽省农业科学院水稻研究所,合肥,230031 2. 安徽省农业科学院水稻研究所,合肥,230031 3. 安徽省农业科学院植物保护研究所,合肥,230031 |
| 基金项目： | 国家高技术研究发展计划(863计划);国家转基因植物研究和产业化专项基金;安徽省自然科学基金 |
| 摘 要： | 通过有性杂交和田间多代选育，利用分子标记辅助选择和田间／温室抗性鉴定，将广谱高抗白叶枯病的Xa21，基因和高抗稻瘟病的Pi9(t)基因聚合到同一品系中，获得双基因纯合且农艺性状稳定的株系。用中国7个和安徽省流行白叶枯病病菌以及多个稻瘟病小种进行抗病性鉴定，结果表明：双抗基因系同时抗白叶枯病和稻瘟病，抗性级别为HR-R，抗谱与供体亲本一致，抗性水平基本相当。室内考种结果显示：双抗基因系间株高变异较大；单株有效穗比两亲本弱；结实率和千粒重明显优于Xa21，基因的供体M12，与Pi9(t)基因的供体亲本75-1-127相当。 |
| 关 键 词： | 白叶枯病 稻瘟病 Xa21基因 Pi9(t)基因 分子标记辅助选择 |
Pyramiding Xa21 and Pi9(t) in Rice by Marker-Assisted Selection |
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| Ni Dahu,Yi Chenxing,Li Li,Wang Xiufeng,Wang Wenxiang,Yang Jianbo.Pyramiding Xa21 and Pi9(t) in Rice by Marker-Assisted Selection[J].Molecular Plant Breeding,2005,3(3):329-334. | |
| Authors: | Ni Dahu Yi Chenxing Li Li Wang Xiufeng Wang Wenxiang Yang Jianbo |
| Abstract: | The homozygous lines which conferred the broad spectrum and high resist ance genes Xa21 and Pi9(t) and had stable agronomic characters were obtained by marker-assisted selection (MAS), successively agronomic selection and inoculatio n of pathogen Xoo and blast in the field /greenhouse. According to the performan ce of artificial inoculation of multiple strains in the field the lines expresse d high resistance to bacterial blight and blast and had same spectrum and simila r resistant level as donor varieties. The assay of agronomic characters displaye d that the plant height varied greatly and the productive tiller numbers per pla nt were lower than donor parents. But fertility and 1 000 grains weight were bet ter than M12 (donor of Xa21) and similar with 75-1-127 (donor of Pi9(t)). |
| Keywords: | Bacterial blight Blast Gene Xa21 Gene Pi9(t) Marker-assisted selection |
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