Rapid extraction, radioiodination, and in vivo catabolism of 125I-labeled fibrinogen in the horse |
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| Authors: | C P Coyne W J Hornof A B Kelly T R O'Brien S J DeNardo |
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| Abstract: | Two methods were analyzed for the rapid extraction of equine fibrinogen from fresh plasma, using ammonium sulfate-sodium phosphate buffer. Fibrinogen from each of these 2 methods was then radiolabeled with 125I (half-life = 60.2 days, gamma = 35 keV), using monochloroiodine reagent. Mean protein-bound activity was 98.5% and mean clottable radioactivity was 94.1%. Radiolabeled fibrinogen administered IV to 15 horses had an overall mean (+/- SD) plasma half-life of 4.95 +/- 0.44 days. |
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