| 同源四倍体高粱与约翰逊草远缘杂种的花药培养初报 |
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| 引用本文： | 蒋观敏,罗耀武,张继益.同源四倍体高粱与约翰逊草远缘杂种的花药培养初报[J].河北农业大学学报,1996(3). |
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| 作者姓名： | 蒋观敏 罗耀武 张继益 |
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| 作者单位： | 河北农业大学农学系 |
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| 摘 要： | 以同源四倍体高粱品系四甜－１、保持系四６２２Ｂ、约翰逊草以及同源四倍体不育系四６２２Ａ×约翰逊草和四甜－１×约翰逊草的杂种后代为材料，分别在１／２ＭＳ、Ｎ６和Ｗ１４培养基上进行花药培养。四６２２Ａ×约翰逊草和四甜－１×约翰逊草的杂种后代在Ｗ１４培养基上产生了愈伤组织，同源四倍体亲本６２２Ｂ在Ｎ６培养基上产生了愈伤组织。对一定的培养基，愈伤组织的诱导主要取决于基因型。另外，对Ｗ１４培养基设置了加活性碳与不加活性碳两组试验，结果表明，活性碳可有效控制花药中产生的有害色素的影响。
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| 关 键 词： | 高梁 约翰逊草 远缘杂交 同源四倍体 花药培养 |
Primary Reports on Anther Culture of Hybrids between Autotetraploid Sorghum and Johnsongrass |
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| Jiang Guanmin, Luo Yoowu,Zhong Jiyi.Primary Reports on Anther Culture of Hybrids between Autotetraploid Sorghum and Johnsongrass[J].Journal of Agricultural University of Hebei,1996(3). |
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| Authors: | Jiang Guanmin Luo Yoowu Zhong Jiyi |
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| Abstract: | Two Lines,including autotetraploid 622B and autotetraploid Tian-1;Johnsongrass (S. halepens) and two hybrids, one autoteraploid 622A×Johnsongrass and one autotetraploid Tian-1×Johnsongrass were tested for their response to anther culture.Among the three media tested (1/2MS, W14, N6), W14 medium was effective in inducing anther calli from the two hybrids while N6 medium was effective in inducing anther calli from autotetraploid 622B. For a given medium, callus induction was obviously genotype dependent. Besides,W14 medium was divided into two groups:one with active carbon and the other without.The result showed that the active carbon could control the influence of the harmful pigment. |
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| Keywords: | Sorghum Johnsongrass(S halepens) Wide hybridization Autotetraploid Anther culture |
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