| 猪巨细胞病毒福建株gB基因的克隆和序列分析 |
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| 引用本文： | 陈如敬,周伦江,吴学敏,车勇良,王晨燕,王隆柏,黄晓凤,严山,刘玉涛,魏宏.猪巨细胞病毒福建株gB基因的克隆和序列分析[J].福建农业学报,2017,32(1). |
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| 作者姓名： | 陈如敬 周伦江 吴学敏 车勇良 王晨燕 王隆柏 黄晓凤 严山 刘玉涛 魏宏 |
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| 作者单位： | 福建省农业科学院畜牧兽医研究所/福建省畜禽疫病防治工程技术研究中心,福建福州,350013 |
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| 基金项目： | 福建省畜禽疫病防控技术重大研发平台项目，福建省科技计划项目——省属公益类科研院所基本科研专项 |
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| 摘 要： | 为明确猪巨细胞病毒福建株(PCMV-FJ01)的gB基因特征,根据其序列特征,设计特异性引物对其进行分段扩增,并对目的片段进行克隆测序。结果发现,PCMV-FJ01株gB基因全长为2 580bp,编码859个氨基酸;其和GenBank数据库中的PCMV分离株的gB基因的核苷酸和氨基酸同源性分别均在98.3%和97.9%以上。此外,本研究还发现部分(约50%)PCMV代表株的gB基因存在ACA三核苷酸缺失(436位氨基酸存在谷氨酰胺Q的缺失)。除ZZ株外,PCMV不同分离株的gB基因是否存在基因缺失和其遗传进化正相关。
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| 关 键 词： | 猪巨细胞病毒 gB基因 克隆 序列分析 |
Cloning and Sequencing of gB Gene from Porcine Cytomegalovirus Isolated in Fujian |
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| CHEN Ru-jing,ZHOU Lun-jiang,WU Xue-min,CHE Yong-liang,WANG Chen-yan,WANG Long-bai,HUANG Xiao-feng,YAN Shan,LIU Yu-tao,WEI Hong.Cloning and Sequencing of gB Gene from Porcine Cytomegalovirus Isolated in Fujian[J].Fujian Journal of Agricultural Sciences,2017,32(1). |
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| Authors: | CHEN Ru-jing ZHOU Lun-jiang WU Xue-min CHE Yong-liang WANG Chen-yan WANG Long-bai HUANG Xiao-feng YAN Shan LIU Yu-tao WEI Hong |
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| Abstract: | Based upon the characterization of gB gene retrieved from GenBank,specific primers were selected to amplify the gB gene of porcine cytomegalovirus (PCMV) FJ01 isolated in Fujian.The target PCR fragments were then cloned and sequenced.The gB gene was found to have 2 580 nucleotides,encoding 859 amino acids.Its homologies on nucleotide and amino acid sequences with the data at GenBank were greater than 98.3 ％ and 97.9 ％,respectively.In addition,nearly half of the PCMV isolates had a same ACA nucleotide deletion in their gB genes that correlated to the phylogenetic evolution of the isolates,except ZZ strain. |
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| Keywords: | porcine cytomegalovirus gB gene clone sequence analysis |
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