| 不同盐度下中华绒螯蟹胁迫相关基因的表达差异 |
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| 引用本文： | 彭智文,黄姝,岳武成,陈晓雯,王军,王成辉.不同盐度下中华绒螯蟹胁迫相关基因的表达差异[J].福建农林大学学报(自然科学版),2017,46(5). |
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| 作者姓名： | 彭智文 黄姝 岳武成 陈晓雯 王军 王成辉 |
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| 作者单位： | 1. 上海海洋大学农业部淡水水产种质资源重点实验室,上海201306;上海海洋大学水产科学国家级实验教学示范中心,上海201306;2. 上海海洋大学上海水产养殖工程技术研究中心,上海,201306;3. 上海海洋大学农业部淡水水产种质资源重点实验室,上海201306;上海海洋大学水产科学国家级实验教学示范中心,上海201306;上海海洋大学上海水产养殖工程技术研究中心,上海201306 |
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| 基金项目： | 上海市科委崇明科技专项项目，上海市工程技术中心建设项目 |
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| 摘 要： | 通过对中华绒螯蟹在4个盐度梯度(0、10、20、30)下的胁迫试验,观察了4个类别的10个相关基因,即4个能量代谢相关基因、3个生长相关基因、2个应激性相关基因和1个渗透压调节相关基因的表达情况.结果表明:3个能量代谢相关基因(VATB、UBE、GAPDH)、1个应激性相关基因(GST)和渗透压调节相关基因(NAK)在不同盐度间的表达量存在显著差异(P0.05),而其他基因的表达量差异不显著(P0.05).应用Best Keeper、Norm Finder和Ge Norm 3个软件对各基因的稳定性进行分析发现:应激性相关基因(GST)和渗透压调节相关基因(NAK)对盐度变化的响应程度较高,表达不稳定;3个生长相关基因(S27、β-ACTIN、α-TUB)对盐度变化的响应程度低,表达稳定,可以作为不同盐度处理下基因差异表达的内参基因.
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| 关 键 词： | 中华绒螯蟹 盐胁迫 功能基因 表达稳定性 |
Effect of different salinity on the expression profiles of stress-relative genes in Chinese mitten crab,Eriocheir sinensis |
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| PENG Zhiwen,HUANG Shu,YUE Wucheng,CHEN Xiaowen,WANG Jun,WANG Chenghui.Effect of different salinity on the expression profiles of stress-relative genes in Chinese mitten crab,Eriocheir sinensis[J].Journal of Fujian Agricultural and Forestry University,2017,46(5). |
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| Authors: | PENG Zhiwen HUANG Shu YUE Wucheng CHEN Xiaowen WANG Jun WANG Chenghui |
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| Abstract: | The expression profiles of 10 stress-related genes were investigated in Eriocheir sinensis under 4 salinity stress ( 0, 10, 20, 30) . The targeted genes included 4 energy metabolism genes ( vacuolar ATPsynthase subunit B, VATB;ubiquitin-conjugating en-zyme E2b, UBE;arginine kinase, AK;glyceraldehyde-3-phosphate dehydrogenase, GAPDH) , 3 growth related genes ( ubiquitin/ri-bosomal S27 fusionprotein, S27; beta-actin, β-ACTIN; alpha-tubulin, α-TUB ) , 2 stress related genes ( heat shock protein90, HSP90; glutathione S-transferase, GST) , and one osmotic regulation gene ( sodium-potassium ATPase alpha subunit, NAK) . The re-sults showed expression varied significantly among 3 energy metabolism related genes ( VATB, UBE, GAPDH) , and stress related gene of GST and osmoregulation gene of NAK) under different salinity stress ( P<0.05) , whereas no significant expression difference among the other genes( P>0.05) . Referring to BestKeeper, NormFinder and GeNorm, expressions of GST and NAK were sensitive and instable under different salinity stress, but expressions of S27, β-ACTIN and α-TUB) were highly stable, implying these 3 genes could be reference genes in expression analysis of Chinese mitten crab under salinity stress. |
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| Keywords: | Eriocheir sinensis salinity stress function gene expression stability |
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