| 水晶花烛的无菌播种和组培快繁技术研究 |
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| 引用本文： | 林辉锋,尚伟,陈昌铭,林发壮,夏朝水,姚凤琴,周辉明.水晶花烛的无菌播种和组培快繁技术研究[J].福建农业学报,2017,32(3). |
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| 作者姓名： | 林辉锋 尚伟 陈昌铭 林发壮 夏朝水 姚凤琴 周辉明 |
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| 作者单位： | 福建省三明市农业科学研究院,福建 沙县,365051 |
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| 摘 要： | 无菌播种水晶花烛Anthurium crystallinum种子获得无菌苗,取该无菌苗的带节茎段、叶片、叶柄为外植体,研究不同诱导培养基组成对水晶花烛愈伤组织诱导分化的影响,同时对影响水晶花烛芽体增殖和壮苗生根的主要因子进行研究。结果表明:水晶花烛种子较好的灭菌方法:种子经去果皮处理+0.1%升汞消毒8min;外植体愈伤诱导分化以带节茎段为宜,培养基为1/3MS+6-BA1.0mg·L~(-1)+2,4-D 0.3mg·L~(-1);芽体增殖培养基:MS+6-BA 2.0mg·L~(-1)+NAA 0.3mg·L~(-1);壮苗生根培养基:MS+NAA 0.2mg·L~(-1),其移栽成活率可达95%以上。
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| 关 键 词： | 水晶花烛 种子处理 无菌播种 组培快繁 |
Aseptic Sowing and Rapid Propagation of Anthurium crystallinum |
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| LIN Hui-feng,SHANG Wei,CHEN Chang-ming,LIN Fa-zhuang,XIA Chao-shui,YAO Feng-qin,ZHOU Hui-ming.Aseptic Sowing and Rapid Propagation of Anthurium crystallinum[J].Fujian Journal of Agricultural Sciences,2017,32(3). |
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| Authors: | LIN Hui-feng SHANG Wei CHEN Chang-ming LIN Fa-zhuang XIA Chao-shui YAO Feng-qin ZHOU Hui-ming |
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| Abstract: | Anthurium crystallinum seedlings were aseptically obtained from seeds for the study.Stem segments with node,leaves and petiole were used as plantlets,and the main factors of callus induction and differentiation as well asthe bud proliferation and seedling rooting during the propagating process were investigated.The results showed that theseed disinfection could be satisfactorily achieved by treating the peeledseeds with 0.1% mercuric chloride for 8min;the optimal callus differentiation of theplantlets was to use the stem segment with node,and cultivated in medium containing 1/3 MS+ 6-BA 1.0 mg·L-1+ 2,4-D 0.3 mg·L-1;the appropriate medium forthe bud proliferation was MS+ 6-BA 2.0 mg·L-1+NAA 0.3 mg·L-1;that the medium for optimal rooting was MS+NAA 0.2 mg·L-1;and a transplanting survival rate up to 95% could be achieved as a result. |
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| Keywords: | Anthurium crystallinum seed pre-treatment aseptic sowing rapid propagation |
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