| 红掌愈伤组织辐射诱变及突变体的筛选 |
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| 引用本文： | 尚伟,李齐向,周辉明,陈昌铭,林发壮,江秋萍.红掌愈伤组织辐射诱变及突变体的筛选[J].福建农业学报,2017,32(7). |
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| 作者姓名： | 尚伟 李齐向 周辉明 陈昌铭 林发壮 江秋萍 |
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| 作者单位： | 1. 福建省三明市农业科学研究院,福建 三明,365051;2. 三明市农业局,福建 三明,365000 |
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| 摘 要： | 利用~(60)Co-γ射线对红掌‘阿拉巴马’的愈伤组织进行辐射,根据再生植株的叶色和花色变化筛选出突变体,并对突变体与亲本进行形态学、细胞学及分子水平的差异分析。结果表明:红掌‘阿拉巴马’愈伤组织的半致死剂量接近30Gy。当辐射剂量为30Gy时,再生植株M_1代、M_2代的性状一致,染色体数目与亲本一致2n=2x=30,仅有RAPD-751引物(序列:CCCACCACAC)条带与亲本有差异,并且能够稳定遗传,从而成功诱变出红掌突变体,这对提高红掌的育种效率具有重要的理论和实践意义。
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| 关 键 词： | 红掌‘阿拉巴马’ 愈伤组织 60Coγ射线 辐射诱变 突变体 |
Radiation-induced Mutation and Mutant Screening on Calluses of Anthurium andraeanum |
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| SHANG Wei,LI Qi-xiang,ZHOU Hui-Ming,CHEN Chang-ming,LIN Fa-zhuang,JIANG Qiu-Ping.Radiation-induced Mutation and Mutant Screening on Calluses of Anthurium andraeanum[J].Fujian Journal of Agricultural Sciences,2017,32(7). |
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| Authors: | SHANG Wei LI Qi-xiang ZHOU Hui-Ming CHEN Chang-ming LIN Fa-zhuang JIANG Qiu-Ping |
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| Abstract: | Calluses from Anthurium andraeanum (Alabama) were exposed to 60Co-γ rays to induce mutation on the tissue.Based on the color changes on the leaves and flowers,resulting mutants were selected among the regenerated plants.Subsequently,the morphological,cellular and molecular properties of the selected mutants and their parents were analyzed.The half-lethal dose of 60Co γ-ray on the callus was found to be close to 30 Gy.Treated by the dosage,the mutated M1 and M2 generations showed similar traits.They had a chromosome number same as that of the parents.But, they differed from the parents in RAPD on the D-751 primer that had a sequence of CCCACCACAC.Since the mutants could consistently pass on the inherited genes to the regenerated protoplasts,theoretically,successful breeding of mutated plants from A.andraeanum by irradiation treatment could be expected in practice. |
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| Keywords: | Anthurium alabama callus 60Co γ-ray radiation-induced mutant |
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