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3种诱抗剂对凤丹抗病害的诱导效果
引用本文:缪福俊,安曼云,华梅,王毅,原晓龙,陈剑,王娟.3种诱抗剂对凤丹抗病害的诱导效果[J].福建农林大学学报(自然科学版),2017,46(3).
作者姓名:缪福俊  安曼云  华梅  王毅  原晓龙  陈剑  王娟
作者单位:1. 云南省林业科学院,云南省森林植物培育与开发利用重点实验室/国家林业局云南珍稀濒特森林植物保护和繁育重点实验室,云南 昆明650201;2. 云南经济管理学院工程学院,云南 安宁,650106
基金项目:国家林业公益行业科研专项,云南省科技计划项目,云南省森林植物培育与开发利用重点实验室/国家林业局云南珍稀濒特植物保护与繁育重点实验室自主创新项目,云南省中青年学术技术带头人后备人才培养项目
摘    要:分别用5个浓度的3种诱抗剂(Harpin、BTH和SA)喷施凤丹叶面,并以清水处理为对照,统计叶片病斑面积和病叶数,从而测定诱抗剂对凤丹抗3种主要病害(红斑病、灰霉病和黑斑病)的诱导效果;同时,测定了最适浓度诱抗剂对3种病原菌的抑制活性,以及对凤丹植株生理指标的影响.结果表明:3种诱抗剂均可有效地诱导凤丹对不同病害产生抗性,其中,BTH的诱抗效果最佳;3种诱抗剂对凤丹抗主要病害的诱导效果均表现为红斑病黑斑病灰霉病;诱抗剂处理显著提高了凤丹植株可溶性蛋白和色素含量及相关酶活性,显著降低了超氧阴离子自由基和丙二醛的含量;诱抗剂对凤丹3种病害的病原菌无毒杀作用,但可诱导植株获得系统抗性.

关 键 词:凤丹  诱抗剂  诱抗效果  系统获得性抗性

Effect of induced resistance to diseases of Paeonia ostii using 3 inducers
MIAO Fujun,AN Manyun,HUA Mei,WANG Yi,YUAN Xiaolong,CHEN Jian,WANG Juan.Effect of induced resistance to diseases of Paeonia ostii using 3 inducers[J].Journal of Fujian Agricultural and Forestry University,2017,46(3).
Authors:MIAO Fujun  AN Manyun  HUA Mei  WANG Yi  YUAN Xiaolong  CHEN Jian  WANG Juan
Abstract:Three inducers (Harpin, BTH and SA) were sprayed to Paeonia ostii in 5 concentrations, with water spray being the control, total spot area and the number of infected leaves were recorded. Meanwhile, at the optimal concentration of inducers, plaque diameter of Cladosporium paeoniae, Botrytis paeoniae and Alternaria alternate were compared, key biochemical indicators re-lated to infections were tested. The results showed that all 3 inducers had significant induction effect on P. ostii resistance to patho-genic fungi, with BTH being the optimal inducer. Averagely induction effect of resistance to fungi in descending order was C.paeoni-ae, A.alternata, and B.paeoniae. Superoxide anion free radical and malondialdehyde levels declined dramatically while levels of sol-uble protein and pigments, and enzymes activity increased compared with the control. In additon, three inducers were not capable of killing pathogens, however, they can stimulate systemic acquired resistance of plant.
Keywords:Paeonia ostii  inducer  induced-resistance effect  systemic acquired resistance
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